ArticleThe Journal of physiology2012
Lifelong physical activity prevents an age-related reduction in arterial and skeletal muscle nitric oxide bioavailability in humans.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 117 citations in OpenAlex.
- Short-term beetroot juice supplementation improves muscle speed and power but does not reduce blood pressure or oxidative stress in 65-79 y old men and women.Nitric oxide : biology and chemistry · 2023Trial
- Effect of Acute Dietary Nitrate Supplementation on the Venous Vascular Response to Static Exercise in Healthy Young Adults.Nutrients · 2022Trial
- Simultaneous Pharmacokinetic Analysis of Nitrate and its Reduced Metabolite, Nitrite, Following Ingestion of Inorganic Nitrate in a Mixed Patient Population.Pharmaceutical research · 2020Trial
- Dietary nitrate does not acutely enhance skeletal muscle blood flow and vasodilation in the lower limbs of older adults during single-limb exercise.European journal of applied physiology · 2020Trial
- A Single Dose of Dietary Nitrate Increases Maximal Knee Extensor Angular Velocity and Power in Healthy Older Men and Women.The journals of gerontology. Series A, Biological sciences and medical sciences · 2020Trial
- Folic acid ingestion improves skeletal muscle blood flow during graded handgrip and plantar flexion exercise in aged humans.American journal of physiology. Heart and circulatory physiology · 2017Trial
- Acute effect of dietary nitrate on forearm muscle oxygenation, blood volume and strength in older adults: A randomized clinical trial.PloS one · 2017Trial
- Acute supplementation of N-acetylcysteine does not affect muscle blood flow and oxygenation characteristics during handgrip exercise.Physiological reports · 2016Trial
- Effect of antioxidants on histamine receptor activation and sustained postexercise vasodilatation in humans.Experimental physiology · 2015Trial
- Cardiorespiratory function associated with dietary nitrate supplementation.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2014Trial
- Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Trial
- Resveratrol blunts the positive effects of exercise training on cardiovascular health in aged men.The Journal of physiology · 2013Trial
- From "time is brain" to "time is collaterals": updates on the role of cerebral collateral circulation in stroke.Journal of thrombosis and thrombolysis · 2025Review
- Declining nitric oxide bioavailability in cardiovascular aging: mechanistic insights and emerging interventions.The journal of cardiovascular aging · 2025Article
- FAPs orchestrate homeostasis of muscle physiology and pathophysiology.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Review
- The cardiovascular changes underlying a low cardiac output with exercise in patients with type 2 diabetes mellitus.Frontiers in physiology · 2024Review
- Ageing and endothelium-mediated vascular dysfunction: the role of the NADPH oxidases.The Journal of physiology · 2023Article
- The Impact of Training on the Loss of Cardiorespiratory Fitness in Aging Masters Endurance Athletes.International journal of environmental research and public health · 2022Review
- The impact of different forms of exercise on endothelial progenitor cells in healthy populations.European journal of applied physiology · 2022Review
- Pre-stroke Physical Activity and Cerebral Collateral Circulation in Ischemic Stroke: A Potential Therapeutic Relationship?Frontiers in neurology · 2022Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ageing has been proposed to be associated with increased levels of reactive oxygen species (ROS) that scavenge nitric oxide (NO). In eight young sedentary (23 ± 1 years; Y), eight older lifelong sedentary (66 ± 2 years; OS) and eight older lifelong physically active subjects (62 ± 2 years; OA), we studied the effect of ROS on systemic and skeletal muscle NO bioavailability and leg blood flow by infusion of the antioxidant N-acetylcysteine (NAC). Infusion of NAC increased the bioavailability of NO in OS, as evidenced by an increased concentration of stable metabolites of NO (NOx) in the arterial and venous circulation and in the muscle interstitium. In OA, infusion of NAC only increased NOx concentrations in venous plasma whereas in Y, infusion of NAC did not affect NOx concentrations. Skeletal muscle protein levels of endothelial and neuronal NO synthase were 32% and 24% higher, respectively, in OA than in OS. Exercise at 12 W elicited a lower leg blood flow response that was associated with a lower leg oxygen uptake in OS than in Y. The improved bioavailability of NO in OS did not increase blood flow during exercise. These data demonstrate that NO bioavailability is compromised in the systemic circulation and in the musculature of sedentary ageing humans due to increased oxidative stress. Lifelong physical activity opposes this effect within the trained musculature and in the arterial circulation. The lower blood flow response to leg exercise in ageing humans is not associated with a reduced NO bioavailability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.