ArticleBlood transfusion = Trasfusione del sangue2012
Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome.
Article in Blood transfusion = Trasfusione del sangue, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Impact of Different Red Blood Cell Storage Solutions and Conditions on Cell Function and Viability: A Systematic Review.Biomolecules · 2024Pooled it
- The resurgence of whole blood transfusion: Evaluating microcirculatory evidence in hemorrhagic trauma care: A review.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2026Review
- Narrative review of strategies for blood product shelf-life extension.Transfusion · 2026Review
- Anti-citrullinated protein antibody detection by hemagglutination.Rheumatology advances in practice · 2025Article
- Assessing the kinetics of oxygen-unloading from red cells using FlowScore, a flow-cytometric proxy of the functional quality of blood.EBioMedicine · 2025Article
- New and emerging technologies for pretransfusion blood quality assessment: A state-of-the-art review.Transfusion · 2024Review
- Transforming research to improve therapies for trauma in the twenty-first century.Critical care (London, England) · 2024Review
- UPLC-MS/MS method for quantitative determination of the advanced glycation endproductsAnalytical methods : advancing methods and applications · 2023Article
- Urea transport in human red blood cells: Donor variation compared to chloride, glucose, and water transport.The Journal of general physiology · 2023Article
- Cyclic Mechanical Stresses Alter Erythrocyte Membrane Composition and Microstructure and Trigger Macrophage Phagocytosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- The Incidence, Degree, and Timing of Hypocalcemia From Massive Transfusion: A Retrospective Review.Cureus · 2022Article
- A deep 96-well plate RBC storage platform for high-throughput screening of novel storage solutions.Frontiers in physiology · 2022Article
- Allogeneic cord blood red blood cells: assessing cord blood unit fractionation and validation.Blood transfusion = Trasfusione del sangue · 2021Article
- Extracellular Vesicles Derived From Platelets, Red Blood Cells, and Monocyte-Like Cells Differ Regarding Their Ability to Induce Factor XII-Dependent Thrombin Generation.Frontiers in cell and developmental biology · 2020Article
- Hemoglobin-based Oxygen Carriers: Current State-of-the-art and Novel Molecules.Shock (Augusta, Ga.) · 2019Review
- Influence of antibiotic treatment on the detection of S. aureus in whole blood following pathogen enrichment.BMC microbiology · 2019Article
- A robust mass spectrometry method for rapid profiling of erythrocyte ghost membrane proteomes.Clinical proteomics · 2018Article
- Whole Blood Storage in CPDA1 Blood Bags Alters Erythrocyte Membrane Proteome.Oxidative medicine and cellular longevity · 2018Article
- Bio-inspired nanomedicine strategies for artificial blood components.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2017Review
- Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation.Transfusion · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSAGM is currently the standard additive solution used in Europe, while AS-3 is the third additive solution that has been licensed in the USA, and is also the one used in part of Canada. Although AS-3 is based on a saline-adenine-glucose solution, it also contains citrate and phosphate. Storage of red blood cell concentrates in CPD-SAGM is known to lead to the accumulation of a wide series of storage lesions, including membrane protein fragmentation and vesiculation, as we could previously determine through 2-dimensional gel electrophoresis. MATERIALS AND
methodsThrough 2D-SDS-IEF-polyacrilamide gel electrophoresis we performed a time course analysis (day 0, 21 and 42 of storage) of red blood cell membranes from leukocyte-filtered concentrates either stored in CPD-SAGM or CP2D-AS-3. RESULTS AND DISCUSSION: From the present study it emerges that the membrane protein profile of red blood cells stored in presence of AS-3 appears to be slightly different from (better than) previous reports on SAGM-stored counterparts. However, the increase of total membrane spot number due to the presence of fragments at day 21 and the significant decrease at day 42 are suggestive of a universal phenomenon which is not efficiently tackled by either of the two additive solutions investigated in the present study.
conclusionTo further delve into the storage lesion issue for RBCs stored in AS-3, it would be interesting in the future to assay metabolic changes over storage progression as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.