ArticleJournal of neuroscience methods2012
Optogenetic approaches to characterize the long-range synaptic pathways from the hypothalamus to brain stem autonomic nuclei.
Article in Journal of neuroscience methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- Oxytocin and Vasopressin Cross Talk Within the Brain Increases Blood Pressure.Circulation research · 2026Article
- A paraventricular hypothalamic control of social stress susceptibility by targeting brown adipose tissue β3 adrenoceptor.Communications biology · 2026Article
- Development of the neurohypophysis: A major neuroendocrine interface.Journal of neuroendocrinology · 2026Review
- Oxytocin Receptor Expression and Activation in Parasympathetic Brainstem Cardiac Vagal Neurons.eNeuro · 2025Article
- Optogenetic Activation of Intrinsic Cardiac Autonomic Neurons in Excised Perfused Mouse Hearts.Journal of visualized experiments : JoVE · 2025Article
- Molecular and cellular neurocardiology in heart disease.The Journal of physiology · 2025Review
- Insulin activates parasympathetic hepatic-related neurons of the paraventricular nucleus of the hypothalamus through mTOR signaling.Journal of neurophysiology · 2025Article
- Prefrontal control of superior colliculus modulates innate escape behavior following adversity.Nature communications · 2024Article
- Ventral tegmental area glutamate neurons establish a mu-opioid receptor gated circuit to mesolimbic dopamine neurons and regulate opioid-seeking behavior.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Outcomes of hypothalamic oxytocin neuron-driven cardioprotection after acute myocardial infarction.Basic research in cardiology · 2023Article
- Oxytocin mediated excitation of hypoglossal motoneurons: implications for treating obstructive sleep apnea.Sleep · 2023Article
- Hypothalamic Oxytocin Neuron Activation Attenuates Intermittent Hypoxia-Induced Hypertension and Cardiac Dysfunction in an Animal Model of Sleep Apnea.Hypertension (Dallas, Tex. : 1979) · 2023Article
- Acute deep brain stimulation of the paraventricular nucleus of the hypothalamus increases brown adipose tissue thermogenesis in rats.Neuroscience letters · 2023Article
- Local production of corticotropin-releasing hormone in prefrontal cortex modulates male-specific novelty exploration.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- GABA facilitates spike propagation through branch points of sensory axons in the spinal cord.Nature neuroscience · 2022Article
- Targeting Parasympathetic Activity to Improve Autonomic Tone and Clinical Outcomes.Physiology (Bethesda, Md.) · 2022Review
- Sex Differences in the Hypothalamic Oxytocin Pathway to Locus Coeruleus and Augmented Attention with Chemogenetic Activation of Hypothalamic Oxytocin Neurons.International journal of molecular sciences · 2021Article
- Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Novel approaches to restore parasympathetic activity to the heart in cardiorespiratory diseases.American journal of physiology. Heart and circulatory physiology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Recent advances in optogenetic methods demonstrate the feasibility of selective photoactivation at the soma of neurons that express channelrhodopsin-2 (ChR2), but a comprehensive evaluation of different methods to selectively evoke transmitter release from distant synapses using optogenetic approaches is needed. Here we compared different lentiviral vectors, with sub-population-specific and strong promoters, and transgenic methods to express and photostimulate ChR2 in the long-range projections of paraventricular nucleus of the hypothalamus (PVN) neurons to brain stem cardiac vagal neurons (CVNs). Using PVN subpopulation-specific promoters for vasopressin and oxytocin, we were able to depolarize the soma of these neurons upon photostimulation, but these promoters were not strong enough to drive sufficient expression for optogenetic stimulation and synaptic release from the distal axons. However, utilizing the synapsin promoter photostimulation of distal PVN axons successfully evoked glutamatergic excitatory post-synaptic currents in CVNs. Employing the Cre/loxP system, using the Sim-1 Cre-driver mouse line, we found that the Rosa-CAG-LSL-ChR2-EYFP Cre-responder mice expressed higher levels of ChR2 than the Rosa-CAG-LSL-ChR2-tdTomato line in the PVN, judged by photo-evoked currents at the soma. However, neither was able to drive sufficient expression to observe and photostimulate the long-range projections to brainstem autonomic regions. We conclude that a viral vector approach with a strong promoter is required for successful optogenetic stimulation of distal axons to evoke transmitter release in pre-autonomic PVN neurons. This approach can be very useful to study important hypothalamus-brainstem connections, and can be easily modified to selectively activate other long-range projections within the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.