ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Phospholipase Cβ1 is linked to RNA interference of specific genes through translin-associated factor X.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Phospholipase Cβ regulates negative associative memory through calcium dependent and independent mechanisms.The Journal of biological chemistry · 2026Article
- Impacts of Globo H Ceramide on Tumor Microenvironment.Advances in experimental medicine and biology · 2026Review
- PLCβ1 by-passes early growth response -1 to induce the differentiation of neuronal cells.Cell death discovery · 2024Article
- Adenosine AJournal of the American Heart Association · 2023Article
- Conformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis.Journal of biomedical science · 2022Article
- Deformation of caveolae impacts global transcription and translation processes through relocalization of cavin-1.The Journal of biological chemistry · 2022Article
- Activation of Gαq sequesters specific transcripts into Ago2 particles.Scientific reports · 2022Article
- The Gαq/phospholipase Cβ signaling system represses tau aggregation.Cellular signalling · 2020Article
- The role of phospholipase Cβ on the plasma membrane and in the cytosol: How modular domains enable novel functions.Advances in biological regulation · 2019Article
- Phospholipase Cβ1 regulates proliferation of neuronal cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Phospholipase Cβ interacts with cytosolic partners to regulate cell proliferation.Advances in biological regulation · 2018Review
- Regulation of the activity of the promoter of RNA-induced silencing, C3PO.Protein science : a publication of the Protein Society · 2017Article
- Phospholipase Cβ-TRAX Association Is Required for PC12 Cell Differentiation.The Journal of biological chemistry · 2016Article
- RNA-induced silencing attenuates G protein-mediated calcium signals.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Phospholipase Cβ connects G protein signaling with RNA interference.Advances in biological regulation · 2016Review
- The Breast Cancer Susceptibility Gene Product (γ-Synuclein) Alters Cell Behavior through its [corrected] Interaction with Phospholipase Cβ.Cellular signalling · 2016Article
- PLC-beta 1 regulates the expression of miR-210 during mithramycin-mediated erythroid differentiation in K562 cells.Oncotarget · 2014Article
- Linking alpha-synuclein properties with oxidation: a hypothesis on a mechanism underling cellular aggregation.Journal of bioenergetics and biomembranes · 2014Review
- Hydrolysis rates of different small interfering RNAs (siRNAs) by the RNA silencing promoter complex, C3PO, determines their regulation by phospholipase Cβ.The Journal of biological chemistry · 2014Article
- Defining the oligomerization state of γ-synuclein in solution and in cells.Biochemistry · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Phospholipase Cβ1 (PLCβ1) is a G-protein-regulated enzyme whose activity results in proliferative and mitogenic changes in the cell. We have previously found that in solution PLCβ1 binds to the RNA processing protein translin-associated factor X (TRAX) with nanomolar affinity and that this binding competes with G proteins. Here, we show that endogenous PLCβ1 and TRAX interact in SK-N-SH cells and also in HEK293 cells induced to overexpress PLCβ1. In HEK293 cells, TRAX overexpression ablates Ca(2+) signals generated by G protein-PLCβ1 activation. TRAX plays a key role in down-regulation of proteins by small, interfering RNA, and PLCβ1 overexpression completely reverses the 2- to 4-fold down-regulation of GAPDH by siRNA in HEK293 and HeLa cells as seen by an ∼4-fold recovery in both the transcript and protein levels. Also, down-regulation of endogenous PLCβ1 in HEK293 and HeLa cells allows for an ∼20% increase in siRNA(GAPDH) silencing. While PLCβ1 overexpression results in a 50% reversal of cell death caused by siRNA(LDH), it does not affect cell survival or silencing of other genes (e.g., cyclophilin, Hsp90, translin). PLCβ1 overexpression in HEK293 and HeLa cells causes a 30% reduction in the total amount of small RNAs. LDH and GAPDH are part of a complex that promotes H2B synthesis that allows cells to progress through the S phase. We find that PLCβ1 reverses the cell death and completely rescues H2B levels caused by siRNA knockdown of LDH or GAPDH. Taken together, our study shows a novel role of PLCβ1 in gene regulation through TRAX association.
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