ArticleBMC microbiology2012
Acid stress response and protein induction in Campylobacter jejuni isolates with different acid tolerance.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Campylobacter jejuni strains isolated in Brazil have important virulence related genes and survive to different stress conditions.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Lactococcus cremoris decreases virulence‑associated properties, and modifies the protein profile of foodborne pathogen Campylobacter jejuni in biofilm.Gut pathogens · 2026Article
- Characterization and immunoprotection of thioredoxin reductase TrxB knockout mutant ofFrontiers in cellular and infection microbiology · 2025Article
- Pathogenicity and virulence ofVirulence · 2024Review
- The Efficacy of a Ferric Sillen Core-Linked Polymer in Suppressing the Pathogenicity ofAnimals : an open access journal from MDPI · 2024Article
- Insights on the genomic diversity, virulence and resistance profile of a Campylobacter jejuni strain isolated from a hospitalized patient in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Proteome profiling of Campylobacter jejuni 81-176 at 37 °C and 42 °C by label-free mass spectrometry.BMC microbiology · 2024Article
- Roles of viable but non-culturable state in the survival ofFrontiers in cellular and infection microbiology · 2023Article
- Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility.Poultry science · 2022Article
- Roles of Aerotolerance, Biofilm Formation, and Viable but Non-Culturable State in the Survival ofMicroorganisms · 2022Review
- Correlation between Multilocus Sequence Typing and Antibiotic Resistance, Virulence Potential ofFoods (Basel, Switzerland) · 2022Article
- Agents of Campylobacteriosis in Different Meat Matrices in Brazil.International journal of environmental research and public health · 2022Article
- Campylobacter coli strains from Brazil can invade phagocytic and epithelial cells and induce IL-8 secretion.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- The Anti-Microorganisms · 2019Article
- Inhibition and Interactions ofMicroorganisms · 2019Article
- Proteome Profiling by Label-Free Mass Spectrometry Reveals Differentiated Response of Campylobacter jejuni 81-176 to Sublethal Concentrations of Bile Acids.Proteomics. Clinical applications · 2019Article
- Bacillus megaterium adapts to acid stress condition through a network of genes: Insight from a genome-wide transcriptome analysis.Scientific reports · 2018Article
- Article
- The Glutaredoxin Gene,Frontiers in microbiology · 2018Article
- The Campylobacter jejuni Ferric Uptake Regulator Promotes Acid Survival and Cross-Protection against Oxidative Stress.Infection and immunity · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring the transmission route from poultry to the human host, the major foodborne pathogen C. jejuni may experience many types of stresses, including low pH caused by different acids. However, not all strains are equally sensitive to the stresses. The aim of this study was to investigate the response to acid stress of three sequenced C. jejuni strains with different acid tolerances using HCl and acetic acid.
resultsTwo-dimensional gel electrophoresis was used for proteomic analysis and proteins were radioactively labelled with methionine to identify proteins only related to acid exposure. To allow added radioactive methionine to be incorporated into induced proteins, a modified chemically defined broth was developed with the minimal amount of methionine necessary for satisfactory growth of all strains. Protein spots were analyzed using image software and identification was done with MALDI-TOF-TOF. The most acid-sensitive isolate was C. jejuni 327, followed by NCTC 11168 and isolate 305 as the most tolerant. Overall, induction of five proteins was observed within the pI range investigated: 19 kDa periplasmic protein (p19), thioredoxin-disulfide (TrxB), a hypothetical protein Cj0706 (Cj0706), molybdenum cofactor biosynthesis protein (MogA), and bacterioferritin (Dps). Strain and acid type dependent differences in the level of response were observed. For strain NCTC 11168, the induced proteins and the regulator fur were analysed at the transcriptomic level using qRT-PCR. In this transcriptomic analysis, only up-regulation of trxB and p19 was observed.
conclusionsA defined medium that supports the growth of a range of Campylobacter strains and suitable for proteomic analysis was developed. Mainly proteins normally involved in iron control and oxidative stress defence were induced during acid stress of C. jejuni. Both strain and acid type affected sensitivity and response.
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