ArticleClinical and experimental gastroenterology2012
Polyethylene glycol versus dual sugar assay for gastrointestinal permeability analysis: is it time to choose?
Article in Clinical and experimental gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Gut Permeability in Very Low Birth Weight Infants
Gut Permeability and Microbiome in Very Low Birth Weight Preterm Neonates.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- The Effects of Exercise on Indirect Markers of Gut Damage and Permeability: A Systematic Review and Meta-analysis.Sports medicine (Auckland, N.Z.) · 2021Pooled it
- Randomised clinical study: Aspergillus niger-derived enzyme digests gluten in the stomach of healthy volunteers.Alimentary pharmacology & therapeutics · 2015Trial
- Oral absorption of high molecular weight polyethylene glycols: mechanistic, safety and regulatory insights.Frontiers in drug safety and regulation · 2026Review
- Looking Inside of the Intestinal Permeability Regulation by Protein-Derivatives from Bovine Milk.Molecular nutrition & food research · 2024Review
- Intestinal Permeability In Subjects With Rheumatoid Arthritis: A Critical Therapeutic Priority.Integrative medicine (Encinitas, Calif.) · 2024Review
- Breaking the Barrier: The Role of Gut Epithelial Permeability in the Pathogenesis of Hypertension.Current hypertension reports · 2024Review
- Article
- Quantification of Polyethylene Glycol 400 Excreted in the Urine by MALDI-TOF Mass Spectrometry.Pharmaceutics · 2022Article
- Altered Gut Microbiome and Fecal Immune Phenotype in Early Preterm Infants With Leaky Gut.Frontiers in immunology · 2022Article
- The Gastrointestinal Exertional Heat Stroke Paradigm: Pathophysiology, Assessment, Severity, Aetiology and Nutritional Countermeasures.Nutrients · 2020Review
- Noninvasive Biomarkers of Gut Barrier Function in Patients Suffering from Diarrhea Predominant-IBS: An Update.Disease markers · 2020Review
- Noninvasive biomarkers of gut barrier function identify two subtypes of patients suffering from diarrhoea predominant-IBS: a case-control study.BMC gastroenterology · 2018Article
- Microbial Biomarkers of Intestinal Barrier Maturation in Preterm Infants.Frontiers in microbiology · 2018Article
- Human small intestine is capable of restoring barrier function after short ischemic periods.World journal of gastroenterology · 2017Article
- Intestinal Barrier Maturation in Very Low Birthweight Infants: Relationship to Feeding and Antibiotic Exposure.The Journal of pediatrics · 2017Article
- Homeostasis of the gut barrier and potential biomarkers.American journal of physiology. Gastrointestinal and liver physiology · 2017Review
- Assessment of the Intestinal Barrier with Five Different Permeability Tests in Healthy C57BL/6J and BALB/cJ Mice.Digestive diseases and sciences · 2016Article
- Hepatic Injury in Nonalcoholic Steatohepatitis Contributes to Altered Intestinal Permeability.Cellular and molecular gastroenterology and hepatology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIncreased intestinal permeability is an important measure of disease activity and prognosis. Currently, many permeability tests are available and no consensus has been reached as to which test is most suitable. The aim of this study was to compare urinary probe excretion and accuracy of a polyethylene glycol (PEG) assay and dual sugar assay in a double-blinded crossover study to evaluate probe excretion and the accuracy of both tests.
methodsGastrointestinal permeability was measured in nine volunteers using PEG 400, PEG 1500, and PEG 3350 or lactulose-rhamnose. On 4 separate days, permeability was analyzed after oral intake of placebo or indomethacin, a drug known to increase intestinal permeability. Plasma intestinal fatty acid binding protein and calprotectin levels were determined to verify compromised intestinal integrity after indomethacin consumption. Urinary samples were collected at baseline, hourly up to 5 hours after probe intake, and between 5 and 24 hours. Urinary excretion of PEG and sugars was determined using high-pressure liquid chromatography-evaporative light scattering detection and liquid chromatography-mass spectrometry, respectively.
resultsIntake of indomethacin increased plasma intestinal fatty acid-binding protein and calprotectin levels, reflecting loss of intestinal integrity and inflammation. In this state of indomethacin-induced gastrointestinal compromise, urinary excretion of the three PEG probes and lactulose increased compared with placebo. Urinary PEG 400 excretion, the PEG 3350/PEG 400 ratio, and the lactulose/rhamnose ratio could accurately detect indomethacin-induced increases in gastrointestinal permeability, especially within 2 hours of probe intake.
conclusionHourly urinary excretion and diagnostic accuracy of PEG and sugar probes show high concordance for detection of indomethacin-induced increases in gastrointestinal permeability. This comparative study improves our knowledge of permeability analysis in man by providing a clear overview of both tests and demonstrates equivalent performance in the current setting.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.