ArticleLasers in surgery and medicine2012
A fluorescence lifetime imaging classification method to investigate the collagen to lipid ratio in fibrous caps of atherosclerotic plaque.
Article in Lasers in surgery and medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Review
- Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions.Journal of biophotonics · 2021Review
- Current Advances in the Diagnostic Imaging of Atherosclerosis: Insights into the Pathophysiology of Vulnerable Plaque.International journal of molecular sciences · 2020Review
- Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging.Annals of biomedical engineering · 2018Article
- Comprehensive intravascular imaging of atherosclerotic plaque in vivo using optical coherence tomography and fluorescence lifetime imaging.Scientific reports · 2018Article
- Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques.Scientific reports · 2018Article
- Imaging the Cardiac Extracellular Matrix.Advances in experimental medicine and biology · 2018Review
- Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe.Journal of biophotonics · 2016Article
- Imaging Fibrosis and Separating Collagens using Second Harmonic Generation and Phasor Approach to Fluorescence Lifetime Imaging.Scientific reports · 2015Article
- Fluorescence Lifetime Imaging Combined with Conventional Intravascular Ultrasound for Enhanced Assessment of Atherosclerotic Plaques: an Ex Vivo Study in Human Coronary Arteries.Journal of cardiovascular translational research · 2015Article
- Fluorescence lifetime imaging and intravascular ultrasound: co-registration study using ex vivo human coronaries.IEEE transactions on medical imaging · 2015Article
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4 authors.
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Abstract
BACKGROUND AND
objectiveThis study describes a novel fluorescence lifetime imaging (FLIM) classification method to determine the ratio of collagen to lipid content in the fibrous cap of atherosclerotic plaques. Additionally, an analytical process to assess risk of plaque rupture based on this ratio is proposed. Collagen to lipid ratio has been shown to be an important parameter to evaluate structural integrity of the fibrous cap. FLIM and other time-resolved fluorescence techniques have recently been applied to the study of atherosclerosis based on the ability to assess biochemical composition. STUDY DESIGN/MATERIALS AND
methodsAutofluorescence of specimens retrieved during carotid endarterectomy procedures was measured through three optical filters, F377: 377/50 nm, F460: 460/66 nm, and F510: 510/84 nm (center wavelength/bandwidth). A Laguerre deconvolution technique was used for the evaluation of fluorescence decay dynamics. The resulting decay parameters (average fluorescence lifetime and 4 Laguerre coefficients at each of the recorded bandwidths) were used for sample characterization. Linear discriminant analysis (LDA) was used to classify each image into collagen or lipid-rich regions based on these parameters. Ultimately, a risk-level was assigned based on the ratio of collagen to lipid on the surface of the fibrous cap.
resultsFLIM images were acquired in 18 carotid plaque specimens at 43 locations. Classification of collagen and lipid-rich regions within the fibrous cap was performed with sensitivity and specificity of 80% and 82%, respectively.
conclusionsResults from this study show that an LDA method of classifying regions of FLIM images of carotid plaque into collagen and lipid-rich regions is capable of being automated and used to rate the risk of plaque rupture based on autofluorescence decay dynamics and without the need for fluorescence intensity or contrast agents.
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