ArticleParasitology research2012
Application of HRM assays with EvaGreen dye for genotyping Giardia duodenalis zoonotic assemblages.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method.Molecular biology reports · 2024Article
- High-Resolution Melting Analysis as an Appropriate Method to Differentiate betweenIranian journal of public health · 2019Article
- Development of TParasitology research · 2017Article
- Comparison and evaluation of four methods for extracting DNA fromJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Wide genetic variations at 18S ribosomal RNA locus of Cyclospora cayetanensis isolated from Egyptian patients using high resolution melting curve.Parasitology research · 2016Article
- High-resolution melting analysis (HRM) for differentiation of four major Taeniidae species in dogs Taenia hydatigena, Taenia multiceps, Taenia ovis, and Echinococcus granulosus sensu stricto.Parasitology research · 2016Article
- PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.Parasitology research · 2016Article
- Predominance of Giardia lamblia assemblage A among iron deficiency anaemic pre-school Egyptian children.Parasitology research · 2016Article
- Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis.Parasitology research · 2015Article
- Development of a rapid HRM genotyping method for detection of dog-derived Giardia lamblia.Parasitology research · 2015Article
- A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A.BioMed research international · 2015Article
- Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- High resolution melting technique for molecular epidemiological studies of cystic echinococcosis: differentiating G1, G3, and G6 genotypes of Echinococcus granulosus sensu lato.Parasitology research · 2013Article
- Identification of Trypanosoma cruzi discrete typing units (DTUs) through the implementation of a high-resolution melting (HRM) genotyping assay.Parasites & vectors · 2013Article
- Genotype Characteristics ofIranian journal of parasitologyArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-resolution melting (HRM) assay was applied to distinguish between Giardia duodenalis assemblages A and B from human and dog feces based on the triosephosphate isomerase gene (tpi). The genomic DNAs were selected from assemblages A (WB) and B (GS) as reference and plasmids were constructed. The reference plasmids and genomic DNAs from 15 Giardia-positive samples were analyzed by HRM assay. This was followed by separate real-time PCR assays specific for assemblages A and B using EvaGreen (EG) to identify PCR products by melting-point analysis. Our results indicate that PCR with HRM in a one-step closed-tube method is a reliable diagnostic method for G. duodenalis zoonotic assemblage identification and more rapid than restriction length polymorphism analysis and direct sequence analysis, HRM is specific, sensitive, reproducible, and rapid. This study is the first use of EG dye for Giardia genotyping. This assay is a promising approach to determine the presence and genotype of Giardia based on a highly variable gene.
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