ArticlePflugers Archiv : European journal of physiology2012
Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques.
Article in Pflugers Archiv : European journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Combined emission ratiometry and motion tracking for optical mapping of contracting hearts: Validation with monophasic action potentials.The Journal of physiology · 2026Article
- Ventricular fibrillation dynamics reveal regional asymmetry in resilience to cardiac arrest and predict clinical outcome.Cardiovascular research · 2026Article
- Stretchable large-area transparent nanowire composite arrays for label-free multimodal interrogation of cardiac physiology.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Simultaneous optical recording of action potentials and calcium transients in cardiac single cells differentiated from type 1 CPVT-iPS cells.Frontiers in physiology · 2025Article
- Recent advances and current limitations of available technology to optically manipulate and observe cardiac electrophysiology.Pflugers Archiv : European journal of physiology · 2023Review
- Near-infrared voltage-sensitive dyes based on chromene donor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A fully-automated low-cost cardiac monolayer optical mapping robot.Frontiers in cardiovascular medicine · 2023Article
- Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals.American journal of physiology. Heart and circulatory physiology · 2022Review
- Methodology for Cross-Talk Elimination in Simultaneous Voltage and Calcium Optical Mapping Measurements With Semasbestic Wavelengths.Frontiers in physiology · 2022Article
- Article
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- A Complete and Low-Cost Cardiac Optical Mapping System in Translational Animal Models.Frontiers in physiology · 2021Article
- Recent progress in optical voltage-sensor technology and applications to cardiac research: from single cells to whole hearts.Progress in biophysics and molecular biology · 2020Review
- Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes.Bioscience reports · 2019Article
- Article
- Lights, camera, path splitter: a new approach for truly simultaneous dual optical mapping of the heart with a single camera.BMC biomedical engineering · 2019Article
- Article
- A new look at the heart-novel imaging techniques.Herzschrittmachertherapie & Elektrophysiologie · 2018Review
- Improvements in Simultaneous Sodium and Calcium Imaging.Frontiers in cellular neuroscience · 2018Article
- Low-Cost Optical Mapping Systems for Panoramic Imaging of Complex Arrhythmias and Drug-Action in Translational Heart Models.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Whole-heart multi-parametric optical mapping has provided valuable insight into the interplay of electrophysiological parameters, and this technology will continue to thrive as dyes are improved and technical solutions for imaging become simpler and cheaper. Here, we show the advantage of using improved 2nd-generation voltage dyes, provide a simple solution to panoramic multi-parametric mapping, and illustrate the application of flash photolysis of caged compounds for studies in the whole heart. For proof of principle, we used the isolated rat whole-heart model. After characterising the blue and green isosbestic points of di-4-ANBDQBS and di-4-ANBDQPQ, respectively, two voltage and calcium mapping systems are described. With two newly custom-made multi-band optical filters, (1) di-4-ANBDQBS and fluo-4 and (2) di-4-ANBDQPQ and rhod-2 mapping are demonstrated. Furthermore, we demonstrate three-parameter mapping using di-4-ANBDQPQ, rhod-2 and NADH. Using off-the-shelf optics and the di-4-ANBDQPQ and rhod-2 combination, we demonstrate panoramic multi-parametric mapping, affording a 360° spatiotemporal record of activity. Finally, local optical perturbation of calcium dynamics in the whole heart is demonstrated using the caged compound, o-nitrophenyl ethylene glycol tetraacetic acid (NP-EGTA), with an ultraviolet light-emitting diode (LED). Calcium maps (heart loaded with di-4-ANBDQPQ and rhod-2) demonstrate successful NP-EGTA loading and local flash photolysis. All imaging systems were built using only a single camera. In conclusion, using novel 2nd-generation voltage dyes, we developed scalable techniques for multi-parametric optical mapping of the whole heart from one point of view and panoramically. In addition to these parameter imaging approaches, we show that it is possible to use caged compounds and ultraviolet LEDs to locally perturb electrophysiological parameters in the whole heart.
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