ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2013
Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- ZoonoticEmerging microbes & infections · 2026Article
- Laboratory diagnosis of pertussis: A survey on provincial public health laboratory methods.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2026Article
- Molecular methods to diagnose pertussis: a case of confusion withASM case reports · 2025Article
- Review
- A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis.Scientific reports · 2023Article
- Evaluation of BioFire Respiratory Panel 2Microbiology spectrum · 2023Article
- AreViruses · 2021Article
- Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens.BMC microbiology · 2021Article
- Low Detection Rate ofOpen forum infectious diseases · 2020Article
- Pathogen analysis of pertussis-like syndrome in children.BMC infectious diseases · 2020Article
- Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco.BMC infectious diseases · 2020Article
- Multicenter Clinical Evaluation of the Automated AriesJournal of clinical microbiology · 2019Article
- Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain.Journal of clinical microbiology · 2019Article
- Bordetella pertussis population dynamics and phylogeny in Japan after adoption of acellular pertussis vaccines.Microbial genomics · 2018Article
- Molecular detection ofIranian journal of microbiology · 2017Article
- Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541.International journal of molecular sciences · 2017Article
- Epidemiology of pertussis in Casablanca (Morocco): contribution of conventional and molecular diagnosis tools.BMC infectious diseases · 2017Article
- Incidence of Severe and Nonsevere Pertussis Among HIV-Exposed and -Unexposed Zambian Infants Through 14 Weeks of Age: Results From the Southern Africa Mother Infant Pertussis Study (SAMIPS), a Longitudinal Birth Cohort Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Follow-Up of External Quality Controls for PCR-Based Diagnosis of Whooping Cough in a Hospital Laboratory Network (Renacoq) and in Other Hospital and Private Laboratories in France.Journal of clinical microbiology · 2016Article
- Protecting Newborns Against Pertussis: Treatment and Prevention Strategies.Paediatric drugs · 2015Review
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular diagnosis of pertussis and parapertussis syndromes is based on the detection of insertion sequences (IS) 481 and 1001, respectively. However, these IS are also detected in the genomes of various Bordetella species, such that they are not specific for either B. pertussis or B. parapertussis. Therefore, we screened the genome of recently circulating isolates of Bordetella species to compare the prevalence of IS481, IS1001 and, also IS1002 with previously published data and to sequence all IS detected. We also investigated whether the numbers of IS481 and IS1001 copies vary in recently circulating isolates of the different Bordetella species. We used the polymerase chain reaction (PCR) method for screening the genome of circulating isolates and to prepare the fragments for sequencing. We used Southern blotting and quantitative real-time PCR for quantification of the numbers of IS. We found no significant diversity in the sequences of the IS harboured in the genomes of the Bordetella isolates screened, except for a 71-nucleotide deletion from IS1002 in B. bronchiseptica. The IS copy numbers in the genome of recently circulating isolates were similar to those in reference strains. Our results confirm that biological diagnosis targeting the IS481 and IS1001 elements are not specific and detect the species B. pertussis, B. holmesii and B. bronchiseptica (IS481), and B. parapertussis and B. bronchiseptica (IS1001).
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