ReviewMethods (San Diego, Calif.)2012
Yeast one-hybrid assays: a historical and technical perspective.
Review in Methods (San Diego, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- HcEcR Regulates Chitin Biosynthesis via 20E Signaling Rather Than Direct Transcriptional Activation in Hyphantria cunea Larvae.Archives of insect biochemistry and physiology · 2026Article
- Article
- Developmental patterning of adipose tissue bybioRxiv : the preprint server for biology · 2026Article
- Yeast One-Hybrid (Y1H) Assay for Single Promoter-Transcription Factor Interaction in Higher Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cardiac enhancers: Gateway to the regulatory mechanisms of heart regeneration.Seminars in cell & developmental biology · 2025Review
- The Sef1 transcription factor interacts with promoters of riboflavin structural genes in Candida famata.FEMS yeast research · 2025Article
- Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast.Current protocols · 2024Article
- Deep mutational scanning quantifies DNA binding and predicts clinical outcomes of PAX6 variants.Molecular systems biology · 2024Article
- The Putative GATA Transcription FactorLife (Basel, Switzerland) · 2024Article
- FsCGBP, a Cutinase G-Box Binding Protein, Regulates the Growth, Development, and Virulence ofJournal of fungi (Basel, Switzerland) · 2024Article
- Protein-Protein Interactions: Yeast Two Hybrid.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Construction of Yeast One-Hybrid Library ofJournal of fungi (Basel, Switzerland) · 2023Article
- Building an improved transcription factor-centered yeast one hybrid system to identify DNA motifs bound by protein comprehensively.BMC plant biology · 2023Article
- Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Methodology for Constructing a Knowledgebase for Plant Gene Regulation Information.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Review
- Research Advances and Prospects of Orphan Genes in Plants.Frontiers in plant science · 2022Review
- bfc, a novel serpent co-factor for the expression of croquemort, regulates efferocytosis in Drosophila melanogaster.PLoS genetics · 2021Article
- DNA-protein interaction studies: a historical and comparative analysis.Plant methods · 2021Review
- Differential Activation of Partially Redundant Δ9 Stearoyl-ACP Desaturase Genes Is Critical for Omega-9 Monounsaturated Fatty Acid Biosynthesis During Seed Development in Arabidopsis.The Plant cell · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an important technique for detecting physical interactions between sequence-specific regulatory transcription factor proteins (TFs) and their DNA target sites. Multiple versions of the Y1H methodology have been developed, each with technical differences and unique advantages. We will discuss several of these technical variations in detail, and also provide some ideas for how Y1H assays can be further improved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.