ArticleVeterinary journal (London, England : 1997)2013
Detection of Streptococcus equi subspecies equi using a triplex qPCR assay.
Article in Veterinary journal (London, England : 1997), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Development and Evaluation of an Indirect ELISA for Strangles Based on an Untagged EQ8-SclF Fusion Protein.Veterinary sciences · 2026Article
- Real-time fluorometric isothermal assays for detection of Streptococcus equi subspecies equi and Streptococcus equi subspecies zooepidemicus in horses: Validation, comparison and evaluation of their clinical application.Australian veterinary journal · 2026Article
- ImmunogenicmSphere · 2025Article
- High-resolution melting curve FRET-qPCR rapidly distinguishesMicrobiology spectrum · 2025Article
- Integrated CRISPR-Cas12a and RAA one-pot visual strategy for the rapid identification ofFrontiers in cellular and infection microbiology · 2025Article
- Development of novel Streptococcus equi vaccines with an assessment of their immunizing potentials and protective efficacies.BMC veterinary research · 2024Article
- Development of a Real-Time Recombinase-Aided Amplification Method for the Rapid Detection ofMicroorganisms · 2024Article
- Molecular and sequencing study and identification of novel SeM-type in beta-hemolytic streptococci involving the upper respiratory tract in Iran.BMC veterinary research · 2023Article
- Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP).Animals : an open access journal from MDPI · 2023Review
- Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi.Equine veterinary journal · 2023Article
- Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop-mediated isothermal nucleic acid amplification microfluidic device.Journal of veterinary internal medicine · 2021Article
- Globetrotting strangles: the unbridled national and international transmission ofMicrobial genomics · 2021Article
- A Novel Real-Time PCR Assay for the Rapid Detection of VirulentFrontiers in veterinary science · 2021Article
- Streptococcus equi Infections in Horses: Guidelines for Treatment, Control, and Prevention of Strangles-Revised Consensus Statement.Journal of veterinary internal medicine · 2018Article
- Defining the ABC of gene essentiality in streptococci.BMC genomics · 2017Article
- Comparison of nasopharyngeal and guttural pouch specimens to determine the optimal sampling site to detect Streptococcus equi subsp equi carriers by DNA amplification.BMC veterinary research · 2017Article
- Molecular characterization of virulence genes of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in equines.Veterinary world · 2016Article
- Streptococcus equi Detection Polymerase Chain Reaction Assay for Equine Nasopharyngeal and Guttural Pouch Wash Samples.Journal of veterinary internal medicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.