ArticleCell reports2012
FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal.
Article in Cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 148 citations in OpenAlex.
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- In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors.PLoS genetics · 2025Article
- From the Making of a Neural Lineage to the Making of a Tumor: Lessons from the "Simple" Drosophila Brain.Advances in experimental medicine and biology · 2025Review
- Indirect neurogenesis in space and time.Nature reviews. Neuroscience · 2024Review
- Orb2 enables rare-codon-enriched mRNA expression during Drosophila neuron differentiation.Nature communications · 2024Article
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- BRCA1 and ELK-1 regulate neural progenitor cell fate in the optic tectum in response to visual experience inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Growth deregulation and interaction with host hemocytes contribute to tumor progression in a Drosophila brain tumor model.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Systematic analysis of YFP traps reveals common mRNA/protein discordance in neural tissues.The Journal of cell biology · 2023Article
- Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila.PLoS biology · 2023Article
- Standardization of Single-Cell RNA-Sequencing Analysis Workflow to Study Drosophila Ovary.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Motor defects in a Drosophila model for spinal muscular atrophy result from SMN depletion during early neurogenesis.PLoS genetics · 2022Article
- Cell-type-specific chromatin occupancy by the pioneer factor Zelda drives key developmental transitions in Drosophila.Nature communications · 2021Article
- Regulation of Neural Stem Cell Competency and Commitment during Indirect Neurogenesis.International journal of molecular sciences · 2021Review
- Article
- DigiTAG-a RNA Sequencing Approach to Analyze Transcriptomes of Rare Cell Populations inBio-protocol · 2020Article
- Understanding microcephaly through the study of centrosome regulation in Drosophila neural stem cells.Biochemical Society transactions · 2020Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well.
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