ArticleMolecular microbiology2012
Functional control of the Candida albicans cell wall by catalytic protein kinase A subunit Tpk1.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 36 citations in OpenAlex.
- HOM2 and TPK1 are associated with Kluyveromyces marxianus growth under high-temperature and reduced-aeration conditions.World journal of microbiology & biotechnology · 2026Article
- Segmental duplications and supernumerary chromosomes drive antifungal drug resistance in Candida auris.Nature communications · 2026Article
- Protein kinase A signaling regulates immune evasion by shaving and concealing fungal β-1,3-glucan.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cas5 Regulates the Exposure of β-Glucan, the Cell Surface Hydrophobicity, and the Expression of Cell Wall Proteins to Remodel theMicroorganisms · 2025Article
- Sdd3 regulates the biofilm formation ofmBio · 2025Article
- Multi-Omics Profiling ofMicroorganisms · 2023Article
- The Cdc25 and Ras1 Proteins ofJournal of fungi (Basel, Switzerland) · 2023Article
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- Kinase Hog1 and Adr1 Opposingly Regulate Haploid Cell Morphology by Controlling Vacuole Size inJournal of fungi (Basel, Switzerland) · 2022Article
- Development and validation of monoclonal antibodies specific for Candida albicans Als2, Als9-1, and Als9-2.PloS one · 2022Article
- Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified FromFrontiers in microbiology · 2022Article
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- CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata.Scientific reports · 2019Article
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- Mitogen activated protein kinases (MAPK) and protein phosphatases are involved inCell surface (Amsterdam, Netherlands) · 2018Article
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- Fungal Biofilms: Inside Out.Microbiology spectrum · 2017Review
- Yeast casein kinase 2 governs morphology, biofilm formation, cell wall integrity, and host cell damage of Candida albicans.PloS one · 2017Article
- Ribosomal protein S6 phosphorylation is controlled by TOR and modulated by PKA in Candida albicans.Molecular microbiology · 2015Article
- Candida albicans Biofilm Development and Its Genetic Control.Microbiology spectrum · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The cyclic AMP protein kinase A pathway governs numerous biological features of the fungal pathogen Candida albicans. The catalytic protein kinase A subunits, Tpk1 (orf19.4892) and Tpk2 (orf19.2277), have divergent roles, and most studies indicate a more pronounced role for Tpk2. Here we dissect two Tpk1-responsive properties: adherence and cell wall integrity. Homozygous tpk1/tpk1 mutants are hyperadherent, and a Tpk1 defect enables biofilm formation in the absence of Bcr1, a transcriptional regulator of biofilm adhesins. A quantitative gene expression-based assay reveals that tpk1/tpk1 and bcr1/bcr1 genotypes show mixed epistasis, as expected if Tpk1 and Bcr1 act mainly in distinct pathways. Overexpression of individual Tpk1-repressed genes indicates that cell surface proteins Als1, Als2, Als4, Csh1 and Csp37 contribute to Tpk1-regulated adherence. Tpk1 is also required for cell wall integrity, but has no role in the gene expression response to cell wall inhibition by caspofungin. Interestingly, increased expression of the adhesin gene ALS2 confers a cell wall defect, as manifested in hypersensitivity to the cell wall inhibitor caspofungin and a shallow cell wall structure. Our findings indicate that Tpk1 governs C. albicans cell wall properties through repression of select cell surface protein genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.