ArticleBMC plant biology2012
Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 60 citations in OpenAlex.
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Characterization and Fine Mapping of the Stay-Green-Related Spot Leaf GeneInternational journal of molecular sciences · 2025Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Modified expression of TaCYP78A5 enhances grain weight with yield potential by accumulating auxin in wheat (Triticum aestivum L.).Plant biotechnology journal · 2022Article
- Virus-Induced Gene Silencing in Wheat and Related Monocot Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Programmed Degradation of Pericarp Cells in Wheat Grains Depends on Autophagy.Frontiers in genetics · 2021Article
- An Efficient Virus-Induced Gene Silencing System for Functional Genomics Research in Walnut (Frontiers in plant science · 2021Article
- WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat.Scientific reports · 2020Article
- A roadmap for gene functional characterisation in crops with large genomes: Lessons from polyploid wheat.eLife · 2020Article
- PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat.Biological research · 2019Article
- Transcriptomic and Metabolomics Analysis of Different Endosperm Region under Nitrogen Treatments.International journal of molecular sciences · 2019Article
- HvWRKY23 regulates flavonoid glycoside and hydroxycinnamic acid amide biosynthetic genes in barley to combat Fusarium head blight.Plant molecular biology · 2019Article
- Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots.Plant molecular biology · 2019Review
- Article
- Deletion of high-molecular-weight glutenin subunits in wheat significantly reduced dough strength and bread-baking quality.BMC plant biology · 2018Article
- An efficient and improved method for virus-induced gene silencing in sorghum.BMC plant biology · 2018Article
- A wheat caffeic acid 3-O-methyltransferase TaCOMT-3D positively contributes to both resistance to sharp eyespot disease and stem mechanical strength.Scientific reports · 2018Article
- Virus-Induced Gene Silencing (VIGS) in Aegilops tauschii and Its Use in Functional Analysis of AetDREB2.Molecular biotechnology · 2018Article
- TaWRKY70 transcription factor in wheat QTL-2DL regulates downstream metabolite biosynthetic genes to resist Fusarium graminearum infection spread within spike.Scientific reports · 2017Article
- Metabolo-transcriptome profiling of barley reveals induction of chitin elicitor receptor kinase gene (HvCERK1) conferring resistance against Fusarium graminearum.Plant molecular biology · 2017Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Barley stripe mosaic virus (BSMV)-based vector has been developed and used for gene silencing in barley and wheat seedlings to assess gene functions in pathogen- or insect-resistance, but conditions for gene silencing in spikes and grains have not been evaluated. In this study, we explored the feasibility of using BSMV for gene silencing in wheat spikes or grains.
resultsApparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after 13 days post inoculation (dpi), and persisted until 30 dpi, while the spikes inoculated with BSMV:00 remained green during the same period. Grains of BSMV:PDS infected spikes also exhibited photobleaching. Molecular analysis indicated that photobleached spikes or grains resulted from the reduction of endogenous PDS transcript abundances, suggesting that BSMV:PDS was able to induce PDS silencing in wheat spikes and grains. Inoculation onto wheat spikes from heading to flowering stage was optimal for efficient silencing of PDS in wheat spikes. Furthermore, we used the BSMV-based system to reduce the transcript level of 1Bx14, a gene encoding for High-molecular-weight glutenin subunit 1Bx14 (HMW-GS 1Bx14), by 97 % in the grains of the BSMV:1Bx14 infected spikes at 15 dpi, compared with that in BSMV:00 infected spikes, and the reduction persisted until at least 25 dpi. The amount of the HMW-GS 1Bx14 was also detectably decreased. The percentage of glutenin macropolymeric proteins in total proteins was significantly reduced in the grains of 1Bx14-silenced plants as compared with that in the grains of BSMV:00 infected control plants, indicating that HMW-GS 1Bx14 is one of major components participating in the formation of glutenin macropolymers in wheat grains.
conclusionThis is one of the first reports of successful application of BSMV-based virus-induced-gene-silencing (VIGS) for gene knockdown in wheat spikes and grains and its application in functional analysis of the 1Bx14 gene. The established BSMV-VIGS system will be very useful in future research on functional analysis of genes contributing to grain quality and the metabolic networks in developing seeds of wheat.
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