ArticleAnalytical chemistry2012
Single-shot characterization of enzymatic reaction constants Km and kcat by an acoustic-driven, bubble-based fast micromixer.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Acoustohydrodynamic micromixers: Basic mixing principles, programmable mixing prospectives, and biomedical applications.Biomicrofluidics · 2024Review
- Manipulation with sound and vibration: A review on the micromanipulation system based on sub-MHz acoustic waves.Ultrasonics sonochemistry · 2023Review
- Towards an active droplet-based microfluidic platform for programmable fluid handling.Lab on a chip · 2023Article
- Comparison of Acoustic Streaming Flow Patterns Induced by Solid, Liquid and Gas Obstructions.Micromachines · 2020Article
- Acoustofluidic methods in cell analysis.Trends in analytical chemistry : TRAC · 2019Article
- Acoustofluidic devices controlled by cell phones.Lab on a chip · 2018Article
- A Review on Micromixers.Micromachines · 2017Review
- Mixing high-viscosity fluids via acoustically driven bubbles.Journal of micromechanics and microengineering : structures, devices, and systems · 2017Article
- Acoustofluidic actuation of in situ fabricated microrotors.Lab on a chip · 2016Article
- Experimental and numerical studies on standing surface acoustic wave microfluidics.Lab on a chip · 2016Article
- Exploring bubble oscillation and mass transfer enhancement in acoustic-assisted liquid-liquid extraction with a microfluidic device.Scientific reports · 2015Article
- Selectively manipulable acoustic-powered microswimmers.Scientific reports · 2015Article
- In situ fabrication of 3D Ag@ZnO nanostructures for microfluidic surface-enhanced Raman scattering systems.ACS nano · 2014Article
- Investigation of acoustic streaming patterns around oscillating sharp edges.Lab on a chip · 2014Article
- An acoustofluidic micromixer via bubble inception and cavitation from microchannel sidewalls.Analytical chemistry · 2014Article
- Article
- Theory and experiment on particle trapping and manipulation via optothermally generated bubbles.Lab on a chip · 2014Article
- Theory and experiment on resonant frequencies of liquid-air interfaces trapped in microfluidic devices.Journal of applied physics · 2013Article
- An acoustofluidic micromixer based on oscillating sidewall sharp-edges.Lab on a chip · 2013Article
- Multifunctional porous silicon nanopillar arrays: antireflection, superhydrophobicity, photoluminescence, and surface-enhanced Raman scattering.Nanotechnology · 2013Article
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Authors and funding
7 authors.
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Abstract
In this work we present an acoustofluidic approach for rapid, single-shot characterization of enzymatic reaction constants K(m) and k(cat). The acoustofluidic design involves a bubble anchored in a horseshoe structure which can be stimulated by a piezoelectric transducer to generate vortices in the fluid. The enzyme and substrate can thus be mixed rapidly, within 100 ms, by the vortices to yield the product. Enzymatic reaction constants K(m) and k(cat) can then be obtained from the reaction rate curves for different concentrations of substrate while holding the enzyme concentration constant. We studied the enzymatic reaction for β-galactosidase and its substrate (resorufin-β-D-galactopyranoside) and found K(m) and k(cat) to be 333 ± 130 μM and 64 ± 8 s(-1), respectively, which are in agreement with published data. Our approach is valuable for studying the kinetics of high-speed enzymatic reactions and other chemical reactions.
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