ArticlePloS one2012
Aberrant promoter methylation and expression of UTF1 during cervical carcinogenesis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Review
- Efficiency of CIN2+ Detection by Thyrotropin-Releasing Hormone (TRH) Site-Specific Methylation.Viruses · 2023Article
- Expression and clinical significance of undifferentiated embryonic cell transcription factor 1 in breast cancer.Translational cancer research · 2023Article
- AQP5-1364A/C Polymorphism AffectsInternational journal of molecular sciences · 2022Article
- An Insight into the Role of UTF1 in Development, Stem Cells, and Cancer.Stem cell reviews and reports · 2021Review
- Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease.Scientific reports · 2021Article
- DNA methylation of a NF-κB binding site in the aquaporin 5 promoter impacts on mortality in sepsis.Scientific reports · 2019Article
- Potential role of cancer stem cells as biomarkers and therapeutic targets in cervical cancer.Cancer reports (Hoboken, N.J.) · 2019Review
- Racial differences in endometrial cancer molecular portraits in The Cancer Genome Atlas.Oncotarget · 2018Article
- Identification ofFrontiers in genetics · 2018Article
- Modulation of DNA methylation by human papillomavirus E6 and E7 oncoproteins in cervical cancer.Oncology letters · 2018Article
- A higher degree of expression of DNA methyl transferase 1 in cervical cancer is associated with poor survival outcome.International journal of women's health · 2017Article
- Integrated analysis of genome-wide DNA methylation and gene expression profiles identifies potential novel biomarkers of rectal cancer.Oncotarget · 2016Article
- Cervical cancer stem cells: opportunities and challenges.Journal of cancer research and clinical oncology · 2015Review
- Expression profile of undifferentiated cell transcription factor 1 in normal and cancerous human epithelia.International journal of experimental pathology · 2014Article
- DNA promoter methylation-dependent transcription of the double C2-like domain β (DOC2B) gene regulates tumor growth in human cervical cancer.The Journal of biological chemistry · 2014Article
- Embryonic stem cell-specific signature in cervical cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Review
- Systems biology approach to stage-wise characterization of epigenetic genes in lung adenocarcinoma.BMC systems biology · 2013Article
- Analysis of the methylation patterns of the p16 INK4A, p15 INK4B, and APC genes in gastric adenocarcinoma patients from a Brazilian population.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2013Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern of stem cell (SC) markers during tumor development. In this study, we were interested to identify SC genes methylation profiles during cervical carcinogenesis. A genome-wide promoter methylation screening revealed a strong hypermethylation of Undifferentiated cell Transcription Factor 1 (UTF1) promoter in cervical cancer in comparison with normal ectocervix. By direct bisulfite pyrosequencing of DNA isolated from liquid-based cytological samples, we showed that UTF1 promoter methylation increases with lesion severity, the highest level of methylation being found in carcinoma. This hypermethylation was associated with increased UTF1 mRNA and protein expression. By using quantitative RT-PCR and Western Blot, we showed that both UTF1 mRNA and protein are present in epithelial cancer cell lines, even in the absence of its two main described regulators Oct4A and Sox2. Moreover, by immunofluorescence, we confirmed the nuclear localisation of UTF1 in cell lines. Surprisingly, direct bisulfite pyrosequencing revealed that the inhibition of DNA methyltransferase by 5-aza-2'-deoxycytidine was associated with decreased UTF1 gene methylation and expression in two cervical cancer cell lines of the four tested. These findings strongly suggest that UTF1 promoter methylation profile might be a useful biomarker for cervical cancer diagnosis and raise the questions of its role during epithelial carcinogenesis and of the mechanisms regulating its expression.
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