ArticlePloS one2012
The colitis-associated transcriptional profile of commensal Bacteroides thetaiotaomicron enhances adaptive immune responses to a bacterial antigen.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
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- Virofree Associates with the Modulation of Gut Microbiomes and Alleviation of DSS-Induced IBD Symptoms in Mice.ACS omega · 2023Article
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- Probiotic-Based Intervention in the Treatment of Ulcerative Colitis: Conventional and New Approaches.Biomedicines · 2022Review
- Dextran Sulphate Sodium Acute Colitis Rat Model: A Suitable Tool for Advancing Our Understanding of Immune and Microbial Mechanisms in the Pathogenesis of Inflammatory Bowel Disease.Veterinary sciences · 2022Article
- Outlook on next-generation probiotics from the human gut.Cellular and molecular life sciences : CMLS · 2022Review
- Intestine-Specific NHE3 Deletion in Adulthood Causes Microbial Dysbiosis.Frontiers in cellular and infection microbiology · 2022Article
- Manipulating resident microbiota to enhance regulatory immune function to treat inflammatory bowel diseases.Journal of gastroenterology · 2020Review
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- Microbial dysbiosis associated with impaired intestinal NaMucosal immunology · 2018Article
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- Influence of Microbiota on Intestinal Immune System in Ulcerative Colitis and Its Intervention.Frontiers in immunology · 2017Review
- Novel Nucleoside Diphosphatase Contributes to Staphylococcus aureus Virulence.The Journal of biological chemistry · 2016Article
- Mucosal Interactions between Genetics, Diet, and Microbiome in Inflammatory Bowel Disease.Frontiers in immunology · 2016Review
- Transcriptome Profiles of Human Lung Epithelial Cells A549 Interacting with Aspergillus fumigatus by RNA-Seq.PloS one · 2015Article
- Identification and Phylogeny of the First T Cell Epitope Identified from a Human Gut Bacteroides Species.PloS one · 2015Article
- Epithelial transport in inflammatory bowel diseases.Inflammatory bowel diseases · 2014Review
- Does the microbiome play a causal role in spondyloarthritis?Clinical rheumatology · 2014Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundInflammatory bowel diseases (IBD) may be caused in part by aberrant immune responses to commensal intestinal microbes including the well-characterized anaerobic gut commensal Bacteroides thetaiotaomicron (B. theta). Healthy, germ-free HLA-B27 transgenic (Tg) rats develop chronic colitis when colonized with complex gut commensal bacteria whereas non-transgenic (nTg) rats remain disease-free. However, the role of B. theta in causing disease in Tg rats is unknown nor is much known about how gut microbes respond to host inflammation.
methodsTg and nTg rats were monoassociated with a human isolate of B. theta. Colonic inflammation was assessed by histologic scoring and tissue pro-inflammatory cytokine measurement. Whole genome transcriptional profiling of B. theta recovered from ceca was performed using custom GeneChips and data analyzed using dChip, Significance Analysis of Microarrays, and Gene Set Enrichment Analysis (GSEA) software. Western Blots were used to determine adaptive immune responses to a differentially expressed B. theta gene.
resultsB. theta monoassociated Tg rats, but not nTg or germ-free controls, developed chronic colitis. Transcriptional profiles of cecal B. theta were significantly different in Tg vs. nTg rats. GSEA revealed that genes in KEGG canonical pathways involved in bacterial growth and metabolism were downregulated in B. theta from Tg rats with colitis though luminal bacterial concentrations were unaffected. Bacterial genes in the Gene Ontology molecular function "receptor activity", most of which encode nutrient binding proteins, were significantly upregulated in B. theta from Tg rats and include a SusC homolog that induces adaptive immune responses in Tg rats.
conclusionsB. theta induces colitis in HLA-B27 Tg rats, which is associated with regulation of bacterial genes in metabolic and nutrient binding pathways that may affect host immune responses. These studies of the host-microbial dialogue may lead to the identification of novel microbial targets for IBD therapies.
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