ArticlePloS one2012
Transcriptome analysis and SSR/SNP markers information of the blunt snout bream (Megalobrama amblycephala).
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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Who cites it
70 citing papers in PubMed, 241 citations in OpenAlex.
- The effect of dietary folic acid on biochemical parameters and gene expression of three heat shock proteins (HSPs) of blunt snout bream (Megalobrama amblycephala) fingerling under acute high temperature stress.Fish physiology and biochemistry · 2017Trial
- Establishment and Polymorphism Analysis of SNP Markers in the Gynogenic Blunt Snout Bream.Biology · 2026Article
- De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Gonads inAnimals : an open access journal from MDPI · 2025Article
- Article
- Protective effect of fructooligosaccharide against oxidative stress and apoptosis induced by Aeromonas hydrophila in Megalobrama amblycephala.BMC genomics · 2024Article
- Identification of sex-specific markers using genome re-sequencing in the blunt snout bream (Megalobrama amblycephala).BMC genomics · 2024Article
- Interactive effects of dietary leucine and isoleucine affect amino acid profile and metabolism through AKT/TOR signaling pathways in blunt snout bream (Megalobrama amblycephala).Fish physiology and biochemistry · 2024Article
- Dietary betaine and/or TMAO affect hepatic lipid accumulation and glycometabolism of Megalobrama amblycephala exposed to a high-carbohydrate diet.Fish physiology and biochemistry · 2024Article
- Genomics and transcriptomics of the Chinese mitten crabs (Eriocheir sinensis).Scientific data · 2023Article
- The Benefits of Nanosized Magnesium Oxide in FishAntioxidants (Basel, Switzerland) · 2023Article
- Single Nucleotide Polymorphism Markers with Applications in Conservation and Exploitation of Aquatic Natural Populations.Animals : an open access journal from MDPI · 2023Review
- Development of Single Nucleotide Polymorphism and Association Analysis with Growth Traits for Black Porgy (Genes · 2022Article
- In Silico Screening and Development of Microsatellite Markers for Genetic Analysis inAnimals : an open access journal from MDPI · 2022Article
- Population Genomics ofBiology · 2022Article
- Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Aquaculture nutrition · 2022Article
- Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels.Scientific reports · 2021Article
- Discovery of alternatively spliced isoforms and long non-coding RNA in full length brain transcriptomes of anadromous Hilsa shad, Tenualosa ilisha (Hamilton, 1822).Molecular biology reports · 2021Article
- Identification and Characterization of Novel circRNAs Involved in Muscle Growth of Blunt Snout Bream (International journal of molecular sciences · 2021Article
- Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844).Molecular biology reports · 2021Article
- High-Carbohydrate Diet Alleviates the Oxidative Stress, Inflammation and Apoptosis ofAntioxidants (Basel, Switzerland) · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlunt snout bream (Megalobrama amblycephala) is an herbivorous freshwater fish species native to China and has been recognized as a main aquaculture species in the Chinese freshwater polyculture system with high economic value. Right now, only limited EST resources were available for M. amblycephala. Recent advances in large-scale RNA sequencing provide a fast, cost-effective, and reliable approach to generate large expression datasets for functional genomic analysis, which is especially suitable for non-model species with un-sequenced genomes. METHODOLOGY AND PRINCIPAL
findingsUsing 454 pyrosequencing, a total of 1,409,706 high quality reads (total length 577 Mbp) were generated from the normalized cDNA of pooled M. amblycephala individuals. These sequences were assembled into 26,802 contigs and 73,675 singletons. After BLAST searches against the NCBI non-redundant (NR) and UniProt databases with an arbitrary expectation value of E(-10), over 40,000 unigenes were functionally annotated and classified using the FunCat functional annotation scheme. A comparative genomics approach revealed a substantial proportion of genes expressed in M. amblycephala tanscriptome to be shared across the genomes of zebrafish, medaka, tetraodon, fugu, stickleback, human, mouse, and chicken, and identified a substantial number of potentially novel M. amblycephala genes. A total number of 4,952 SSRs were found and 116 polymorphic loci have been characterized. A significant number of SNPs (25,697) and indels (23,287) were identified based on specific filter criteria in the M. amblycephala.
conclusionsThis study is the first comprehensive transcriptome analysis for a fish species belonging to the genus Megalobrama. These large EST resources are expected to be valuable for the development of molecular markers, construction of gene-based linkage map, and large-scale expression analysis of M. amblycephala, as well as comparative genome analysis for the genus Megalobrama fish species. The identified SSR and SNP markers will greatly benefit its breeding program and whole genome association studies.
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