ArticlePloS one2012
ZFX controls the self-renewal of human embryonic stem cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
34 citing papers in PubMed, 52 citations in OpenAlex.
- De Novo Heterozygous ZFX Frameshift Variant in a Female With an X-Linked Neurodevelopmental Disorder.American journal of medical genetics. Part A · 2026Article
- Systematic identification of single transcription factor perturbations that drive cellular and tissue rejuvenation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Epigenetic control of metabolic identity across cell types.BMC genomics · 2025Article
- Inference of gene coexpression networks from single-cell transcriptome data based on variance decomposition analysis.Briefings in bioinformatics · 2025Article
- The Multifaceted Roles of Zinc Finger Proteins in Pluripotency and Reprogramming.International journal of molecular sciences · 2025Review
- IGFBP1 is One of 10 Metabolic Factors Predicting Recurrence Free Survival in Gastric Cancer and Promotes Cancer Progression via ZFX-IGFBP1 Axis.OncoTargets and therapy · 2025Article
- Maintenance of stem cell self-renewal by sex chromosomal zinc-finger transcription factors.World journal of methodology · 2024Article
- Molecular and Clinical Spectrum of Primary Hyperparathyroidism.Endocrine reviews · 2023Article
- Transcriptomic profiling of neonatal mouse granulosa cells reveals new insights into primordial follicle activation†.Biology of reproduction · 2022Article
- Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients.Cells · 2021Article
- A flexible repertoire of transcription factor binding sites and a diversity threshold determines enhancer activity in embryonic stem cells.Genome research · 2021Article
- Comprehensive analyses of 723 transcriptomes enhance genetic and biological interpretations for complex traits in cattle.Genome research · 2020Article
- Methyl-indole inhibits pancreatic cancer cell viability by down-regulating ZFX expression.3 Biotech · 2020Article
- Expression and differential regulation of human TERRA at several chromosome ends.RNA (New York, N.Y.) · 2019Article
- Homoharringtonine enhances the effect of imatinib on chronic myelogenous leukemia cells by downregulating ZFX.Molecular medicine reports · 2019Article
- Lipid Deprivation Induces a Stable, Naive-to-Primed Intermediate State of Pluripotency in Human PSCs.Cell stem cell · 2019Article
- Differential Expression Profile of ZFX Variants Discriminates Breast Cancer SubtypesIranian biomedical journal · 2019Article
- Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set.PloS one · 2019Article
- The Transcription Factor Zfx Regulates Peripheral T Cell Self-Renewal and Proliferation.Frontiers in immunology · 2018Article
- Zinc finger protein X-linked promotes expansion of EpCAMMolecular oncology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer great promise in regenerative medicine and disease modeling due to their unlimited self-renewal and broad differentiation capacity. There is evidence that the growth properties and critical signaling pathways differ between murine and human ESCs; therefore, it is essential to perform functional studies to test the putatively conserved mechanisms of pluripotent stem cell self-renewal between species. Previously, we identified the transcription factor Zfx as a key regulator of self-renewal in murine ESCs. Here we extend those findings to human ESCs. ZFX knockdown in hESCs hindered clonal growth and decreased colony size after serial replating. ZFX overexpression enhanced clone formation in the presence of Y-27632, increased colony size at low density and decreased expression of differentiation-related genes in human ESCs. ZFX-overexpressing hESCs resisted spontaneous differentiation but could be directed to differentiate into endodermal and neural cell fates when provided with the appropriate cues. Thus, ZFX acts as a molecular rheostat regulating the balance between self-renewal and differentiation in hESCs, revealing the close evolutionary conservation of the self-renewal mechanisms in murine and human ESCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.