ArticlePloS one2012
Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Machine learning-assisted decoding of temporal transcriptional dynamics via fluorescent timer.Nature communications · 2025Article
- Article
- Selective ablation of thymic and peripheral Foxp3Frontiers in immunology · 2023Article
- Genetic Tools for Analyzing Foxp3Methods in molecular biology (Clifton, N.J.) · 2023Article
- Role of Dynamic Actin Cytoskeleton Remodeling in Foxp3Frontiers in immunology · 2022Article
- The multifaceted Foxp3European journal of immunology · 2020Article
- LKB1 expressed in dendritic cells governs the development and expansion of thymus-derived regulatory T cells.Cell research · 2019Article
- Inducible IL-7 Hyperexpression Influences Lymphocyte Homeostasis and Function and Increases Allograft Rejection.Frontiers in immunology · 2019Article
- Evaluation of the BDCA2-DTR Transgenic Mouse Model in Chronic and Acute Inflammation.PloS one · 2015Article
- Limitations of Foxp3(+) Treg depletion following viral infection in DEREG mice.Journal of immunological methods · 2014Article
- Foxp3(+) regulatory T cells in mouse models of type 1 diabetes.Journal of diabetes research · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD4(+)CD25(+) regulatory T (Treg) cell lineage commitment and expression of the transcription factor Foxp3 can be induced at the CD4(+)CD8(+) double-positive (DP) and CD4(+)CD8(?) single-positive stages of thymic development, as well as in postthymic CD4(+) T cells in peripheral lymphoid tissues. The availability of transgenic mice with Foxp3-dependent fluorochrome reporter gene expression has greatly facilitated studies on the intra- and extrathymic generation of murine Foxp3(+) Treg cells. Here, we performed a comparative analysis of thymic Treg cell development and peripheral compartments of mature Treg cells in various transgenic strains with gene targeted and bacterial artificial chromosome (BAC)-driven Foxp3-fluorochrome expression. These studies revealed a relative deficiency of Foxp3(+) DP thymocytes selectively in mice with targeted insertion of the fluorochrome reporter gene coding sequence into the endogenous Foxp3 gene. While Foxp3 BAC-driven fluorochrome expression in ex vivo CD4(+) T cells was found to faithfully reflect Foxp3 protein expression, we provide evidence that Foxp3 BAC transgenesis can result in sizable populations of Foxp3(+) Treg cells that lack fluorochrome reporter expression. This could be attributed to both timely delayed up-regulation of BAC expression in developing Treg cells and the accumulation of peripheral Foxp3(+) Treg cells with continuous transcriptional inactivity of the Foxp3 BAC transgene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.