ArticlePloS one2012
Discovery of genes related to insecticide resistance in Bactrocera dorsalis by functional genomic analysis of a de novo assembled transcriptome.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 54 citations in OpenAlex.
- Towards holistic insect monitoring: species discovery, description, identification and traits for all insects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
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- Evidence of Adaptive Evolution and Relaxed Constraints in Sex-Biased Genes of South American and West Indies Fruit Flies (Diptera: Tephritidae).Genome biology and evolution · 2018Article
- DIRProt: a computational approach for discriminating insecticide resistant proteins from non-resistant proteins.BMC bioinformatics · 2017Article
- Identification and preliminary characterization of chemosensory perception-associated proteins in the melon fly Bactrocera cucurbitae using RNA-seq.Scientific reports · 2016Article
- Identification of Male- and Female-Specific Olfaction Genes in Antennae of the Oriental Fruit Fly (Bactrocera dorsalis).PloS one · 2016Article
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- De novo transcriptome assembly of Ipomoea nil using Illumina sequencing for gene discovery and SSR marker identification.Molecular genetics and genomics : MGG · 2015Article
- SuRankCo: supervised ranking of contigs in de novo assemblies.BMC bioinformatics · 2015Article
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- A pipeline for the de novo assembly of the Themira biloba (Sepsidae: Diptera) transcriptome using a multiple k-mer length approach.BMC genomics · 2014Article
- Australian endemic pest tephritids: genetic, molecular and microbial tools for improved Sterile Insect Technique.BMC genetics · 2014Review
- On the optimal trimming of high-throughput mRNA sequence data.Frontiers in genetics · 2014Article
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- How functional genomics will impact fruit fly pest control: the example of the Mediterranean fruit fly, Ceratitis capitata.BMC genetics · 2014Review
- Emerging tools for synthetic genome design.Molecules and cells · 2013Review
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- Mining genes involved in insecticide resistance of Liposcelis bostrychophila Badonnel by transcriptome and expression profile analysis.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insecticide resistance has recently become a critical concern for control of many insect pest species. Genome sequencing and global quantization of gene expression through analysis of the transcriptome can provide useful information relevant to this challenging problem. The oriental fruit fly, Bactrocera dorsalis, is one of the world's most destructive agricultural pests, and recently it has been used as a target for studies of genetic mechanisms related to insecticide resistance. However, prior to this study, the molecular data available for this species was largely limited to genes identified through homology. To provide a broader pool of gene sequences of potential interest with regard to insecticide resistance, this study uses whole transcriptome analysis developed through de novo assembly of short reads generated by next-generation sequencing (NGS). The transcriptome of B. dorsalis was initially constructed using Illumina's Solexa sequencing technology. Qualified reads were assembled into contigs and potential splicing variants (isotigs). A total of 29,067 isotigs have putative homologues in the non-redundant (nr) protein database from NCBI, and 11,073 of these correspond to distinct D. melanogaster proteins in the RefSeq database. Approximately 5,546 isotigs contain coding sequences that are at least 80% complete and appear to represent B. dorsalis genes. We observed a strong correlation between the completeness of the assembled sequences and the expression intensity of the transcripts. The assembled sequences were also used to identify large numbers of genes potentially belonging to families related to insecticide resistance. A total of 90 P450-, 42 GST-and 37 COE-related genes, representing three major enzyme families involved in insecticide metabolism and resistance, were identified. In addition, 36 isotigs were discovered to contain target site sequences related to four classes of resistance genes. Identified sequence motifs were also analyzed to characterize putative polypeptide translational products and associate them with specific genes and protein functions.
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