ArticleNucleic acids research2012
Structure of the RNA claw of the DNA packaging motor of bacteriophage Φ29.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Regulation of the ordinal DNA translocation cycle in bacteriophage Φ29 through trans-subunit interactions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A viral genome packaging motor transitions between cyclic and helical symmetry to translocate dsDNA.Science advances · 2021Article
- Article
- How do ADARs bind RNA? New protein-RNA structures illuminate substrate recognition by the RNA editing ADARs.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- NMR Structures and Dynamics in a Prohead RNA Loop that Binds Metal Ions.The journal of physical chemistry letters · 2016Article
- Prohead RNA: a noncoding viral RNA of novel structure and function.Wiley interdisciplinary reviews. RNA · 2016Review
- Article
- Insights into the structure and assembly of the bacteriophage 29 double-stranded DNA packaging motor.Journal of virology · 2014Article
- Revolution rather than rotation of AAA+ hexameric phi29 nanomotor for viral dsDNA packaging without coiling.Virology · 2013Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacteriophage DNA packaging motors translocate their genomic DNA into viral heads, compacting it to near-crystalline density. The Bacillus subtilis phage 29 has a unique ring of RNA (pRNA) that is an essential component of its motor, serving as a scaffold for the packaging ATPase. Previously, deletion of a three-base bulge (18-CCA-20) in the pRNA A-helix was shown to abolish packaging activity. Here, we solved the structure of this crucial bulge by nuclear magnetic resonance (NMR) using a 27mer RNA fragment containing the bulge (27b). The bulge actually involves five nucleotides (17-UCCA-20 and A100), as U17 and A100 are not base paired as predicted. Mutational analysis showed these newly identified bulge residues are important for DNA packaging. The bulge introduces a 33-35° bend in the helical axis, and inter-helical motion around this bend appears to be restricted. A model of the functional 120b pRNA was generated using a 27b NMR structure and the crystal structure of the 66b prohead-binding domain. Fitting this model into a cryo-EM map generated a pentameric pRNA structure; five helices projecting from the pRNA ring resemble an RNA claw. Biochemical analysis suggested that this shape is important for coordinated motor action required for DNA translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.