ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2012
Characterization of mammary cancer stem cells in the MMTV-PyMT mouse model.
Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
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- RANK links senescence to stemness in the mammary epithelia, delaying tumor onset but increasing tumor aggressiveness.Developmental cell · 2021Article
- Annexin A1 Is Required for Efficient Tumor Initiation and Cancer Stem Cell Maintenance in a Model of Human Breast Cancer.Cancers · 2021Article
- Genomics-Guided Drawing of Molecular and Pathophysiological Components of Malignant Regulatory Signatures Reveals a Pivotal Role in Human Diseases of Stem Cell-Associated Retroviral Sequences and Functionally-Active hESC Enhancers.Frontiers in oncology · 2021Article
- Insights from transgenic mouse models of PyMT-induced breast cancer: recapitulating human breast cancer progression in vivo.Oncogene · 2021Review
- E2F1 Drives Breast Cancer Metastasis by Regulating the Target Gene FGF13 and Altering Cell Migration.Scientific reports · 2019Article
- Integrated analyses of murine breast cancer models reveal critical parallels with human disease.Nature communications · 2019Article
- Inhibition of USP2 eliminates cancer stem cells and enhances TNBC responsiveness to chemotherapy.Cell death & disease · 2019Article
- Regulatory T Cells Control the Switch FromFrontiers in immunology · 2019Article
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- Histological subtypes of mouse mammary tumors reveal conserved relationships to human cancers.PLoS genetics · 2018Article
- Fluorescence-Activated Cell Sorting of Murine Mammary Cancer Stem-Like Cell Subpopulations with HIF Activity.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Downregulation of Bmi1 in breast cancer stem cells suppresses tumor growth and proliferation.Oncotarget · 2017Article
- Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells.Molecular cancer · 2017Article
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- The chemokine receptor CCR7 promotes mammary tumorigenesis through amplification of stem-like cells.Oncogene · 2016Article
- TWIST1 and BMI1 in Cancer Metastasis and Chemoresistance.Journal of Cancer · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Breast cancer stem cells, the root of tumor growth, present challenges to investigate: Primary human breast cancer cells are difficult to establish in culture and inconsistently yield tumors after transplantation into immune-deficient recipient mice. Furthermore, there is limited characterization of mammary cancer stem cells in mice, the ideal model for the study of breast cancer. We herein describe a pre-clinical breast cancer stem cell model, based on the properties of cancer stem cells, derived from transgenic MMTV-PyMT mice. Using a defined set of cell surface markers to identify cancer stem cells by flow cytometry, at least four cell populations were recovered from primary mammary cancers. Only two of the four populations, one epithelial and one mesenchymal, were able to survive and proliferate in vitro. The epithelial population exhibited tumor initiation potential with as few as 10 cells injected into syngeneic immune-competent recipients. Tumors initiated from injected cell lines recapitulated the morphological and physiological components of the primary tumor. To highlight the stemness potential of the putative cancer stem cells, B lymphoma Mo-MLV insertion region 1 homolog (Bmi-1) expression was knocked down via shRNA targeting Bmi-1. Without Bmi-1 expression, putative cancer stem cells could no longer initiate tumors, but tumor initiation was rescued with the introduction of a Bmi-1 overexpression vector in the Bmi-1 knockdown cells. In conclusion, our data show that primary mammary cancers from MMTV-PyMT mice contain putative cancer stem cells that survive in culture and can be used to create a model for study of mammary cancer stem cells.
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