ArticleProtoplasma2013
An improved Agrobacterium-mediated transformation of recalcitrant indica rice (Oryza sativa L.) cultivars.
Article in Protoplasma, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- OsELP Mediated Apoplastic Sequestration in Roots Acts as an Arsenic Filter, Limiting Grain Accumulation in Rice.Plant, cell & environment · 2026Article
- Agrobacterium tumefaciens-Mediated Plant Transformation and Gene Editing in Rice.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Identification of QTLs responsible for culturability, and fine-mapping of QTLMolecular breeding : new strategies in plant improvement · 2024Article
- Somatic Embryogenesis andPlants (Basel, Switzerland) · 2024Article
- Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'.Transgenic research · 2023Article
- Comprehensive Transcriptomic and Metabolic Profiling ofInternational journal of molecular sciences · 2023Article
- Overexpression of maize GOLDEN2 in rice and maize calli improves regeneration by activating chloroplast development.Science China. Life sciences · 2023Article
- A tau class GST, OsGSTU5, interacts with VirE2 and modulates the Agrobacterium-mediated transformation in rice.Plant cell reports · 2022Article
- Optimisation of a rapid and efficient transformation protocol for fungal blast-susceptible indica rice cultivars HR-12 and CO-39.Plant biotechnology (Tokyo, Japan) · 2021Article
- OptimizingInternational journal of molecular sciences · 2021Article
- Functional characterization of tau class glutathione-3 Biotech · 2020Article
- A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals.Plant cell, tissue and organ culture · 2020Article
- Infection of Embryonic Callus withInternational journal of molecular sciences · 2019Article
- Improvement of SoybeanInternational journal of molecular sciences · 2018Article
- Improving agroinfiltration-based transient gene expression inPlant methods · 2018Article
- Article
- Effect of antibiotics on callus regeneration during transformation of IR 64 rice.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Article
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11 authors.
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Abstract
Agrobacterium-mediated transformation of indica rice varieties has been quite difficult as these are recalcitrant to in vitro responses. In the present study, we established a high-efficiency Agrobacterium tumefaciens-mediated transformation system of rice (Oryza sativa L. ssp. indica) cv. IR-64, Lalat, and IET-4786. Agrobacterium strain EHA-101 harboring binary vector pIG121-Hm, containing a gene encoding for β-glucuronidase (GUS) and hygromycin resistance, was used in the transformation experiments. Manipulation of different concentrations of acetosyringone, days of co-culture period, bacterial suspension of different optical densities (ODs), and the concentrations of L-cysteine in liquid followed by solid co-culture medium was done for establishing the protocol. Among the different co-culture periods, 5 days of co-culture with bacterial cells (OD600 nm = 0.5-0.8) promoted the highest frequency of transformation (83.04 %) in medium containing L-cysteine (400 mg l(-1)). Putative transformed plants were analyzed for the presence of a transgene through genomic PCR and GUS histochemical analyses. Our results also suggest that different cultural conditions and the addition of L-cysteine in the co-culture medium improve the Agrobacterium-mediated transformation frequencies from an average of 12.82 % to 33.33 % in different indica rice cultivars.
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