ArticleEuropean radiology2013
The high angiogenic activity in very early breast cancer enables reliable imaging with VEGFR2-targeted microbubbles (BR55).
Article in European radiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- GV21 enables ultrasound molecular imaging of tumor cell-associated VEGFR2 for early therapy assessment.Materials today. Bio · 2026Article
- Evaluation of parietal pleural invasion in subpleural lung cancer by VEGFR2 targeted microbubble-based ultrasound molecular imaging.Translational cancer research · 2026Article
- Advances and innovations in ultrasound-based tumor management: current applications and emerging directions.The ultrasound journal · 2025Review
- Programmable ultrasound imaging guided theranostic nanodroplet destruction for precision therapy of breast cancer.Ultrasonics sonochemistry · 2024Article
- BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.International journal of molecular sciences · 2023Article
- VEGFR2 targeted microbubble-based ultrasound molecular imaging improving the diagnostic sensitivity of microinvasive cervical cancer.Journal of nanobiotechnology · 2023Article
- Imaging methods to evaluate tumor microenvironment factors affecting nanoparticle drug delivery and antitumor response.Cancer drug resistance (Alhambra, Calif.) · 2021Article
- Change of Apoptosis and Glucose Metabolism in Lung Cancer Xenografts during Cytotoxic and Anti-Angiogenic Therapy Assessed by Annexin V Based Optical Imaging andContrast media & molecular imaging · 2021Article
- Molecular Ultrasound Imaging.Nanomaterials (Basel, Switzerland) · 2020Review
- Preclinical Molecular Imaging for Precision Medicine in Breast Cancer Mouse Models.Contrast media & molecular imaging · 2019Review
- Tumor angiogenesis targeting and imaging using gold nanoparticle probe with directly conjugated cyclic NGR.RSC advances · 2018Article
- CAIX aptamer-functionalized targeted nanobubbles for ultrasound molecular imaging of various tumors.International journal of nanomedicine · 2018Article
- Improved Sensitivity in Ultrasound Molecular Imaging With Coherence-Based Beamforming.IEEE transactions on medical imaging · 2018Article
- First-in-Human Study of Acoustic Angiography in the Breast and Peripheral Vasculature.Ultrasound in medicine & biology · 2017Article
- Nilotinib Enhances Tumor Angiogenesis and Counteracts VEGFR2 Blockade in an Orthotopic Breast Cancer Xenograft Model with Desmoplastic Response.Neoplasia (New York, N.Y.) · 2017Article
- In vitro characterization and in vivo ultrasound molecular imaging of nucleolin-targeted microbubbles.Biomaterials · 2017Article
- VEGFR2-Targeted Three-Dimensional Ultrasound Imaging Can Predict Responses to Antiangiogenic Therapy in Preclinical Models of Colon Cancer.Cancer research · 2016Article
- In situ validation of VEGFR-2 and α v ß 3 integrin as targets for breast lesion characterization.Angiogenesis · 2016Article
- Low-Dose Molecular Ultrasound Imaging with E-Selectin-Targeted PBCA Microbubbles.Molecular imaging and biology · 2016Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTumour xenografts of well-discernible sizes can be examined well by molecular ultrasound. Here, we investigated whether very early breast carcinomas express sufficient levels of VEGFR2 for reliable molecular ultrasound imaging with targeted microbubbles.
methodsMCF-7 breast cancer xenografts were orthotopically implanted in nude mice (n = 26). Tumours measuring from 4 mm(3) (2 mm diameter) up to 65 mm(3) (5 mm diameter) were examined with automated 3D molecular ultrasound using clinically translatable VEGFR2-targeted microbubbles (BR55). Additionally, the relative tumour blood volume was assessed with non-targeted microbubbles (BR38). In vivo ultrasound data were validated by quantitative immunohistochemistry.
resultsVery small lesions 2 mm in diameter showed the highest binding of VEGFR2-specific microbubbles. In larger tumours significantly less BR55 accumulated (p = 0.023). Nonetheless, binding of VEGFR2-targeted microbubbles was still high enough for imaging. The relative blood volume was comparable at all tumour sizes. Both findings were confirmed by immunohistochemistry. Additionally, a significantly enhanced number of large and mature vessels were detected with increasing tumour size (p < 0.01), explaining the decrease in VEGFR2 expression during tumour growth.
conclusions3D molecular ultrasound using BR55 is very well suited to depicting the angiogenic activity in very small breast lesions, suggesting its potential for detecting and characterising these lesions.
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