ArticleHistochemistry and cell biology2013
A simple method to obtain pure cultures of multiciliated ependymal cells from adult rodents.
Article in Histochemistry and cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells.Fluids and barriers of the CNS · 2026Article
- Gait and posture analysis in rats with Parkinson's disease using DeepLabCut during beam test.Open veterinary journal · 2025Article
- PCD Genes-From Patients to Model Organisms and Back to Humans.International journal of molecular sciences · 2022Review
- IIIG9 inhibition in adult ependymal cells changes adherens junctions structure and induces cellular detachment.Scientific reports · 2021Article
- (Biological psychiatry global open science · 2021Article
- Microglia activated by microbial neuraminidase contributes to ependymal cell death.Fluids and barriers of the CNS · 2021Article
- Preterm intraventricular hemorrhage in vitro: modeling the cytopathology of the ventricular zone.Fluids and barriers of the CNS · 2020Article
- Article
- Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation and Maturation of Ependymal Cells.Molecular neurobiology · 2018Article
- Complement system activation contributes to the ependymal damage induced by microbial neuraminidase.Journal of neuroinflammation · 2016Article
- Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity.Journal of cell science · 2016Article
- DAPT mediates atoh1 expression to induce hair cell-like cells.American journal of translational research · 2016Article
- The Histochem Cell Biol conspectus: the year 2013 in review.Histochemistry and cell biology · 2014Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ependymal cells form an epithelium lining the ventricular cavities of the vertebrate brain. Numerous methods to obtain primary culture ependymal cells have been developed. Most of them use foetal or neonatal rat brain and the few that utilize adult brain hardly achieve purity. Here, we describe a simple and novel method to obtain a pure non-adherent ependymal cell culture from explants of the striatal and septal walls of the lateral ventricles. The combination of a low incubation temperature followed by a gentle enzymatic digestion allows the detachment of most of the ependymal cells from the ventricular wall in a period of 6 h. Along with ependymal cells, a low percentage (less than 6 %) of non-ependymal cells also detaches. However, they do not survive under two restrictive culture conditions: (1) a simple medium (alpha-MEM with glucose) without any supplement; and (2) a low density of 1 cell/µl. This purification method strategy does not require cell labelling with antibodies and cell sorting, which makes it a simpler and cheaper procedure than other methods previously described. After a period of 48 h, only ependymal cells survive such conditions, revealing the remarkable survival capacity of ependymal cells. Ependymal cells can be maintained in culture for up to 7-10 days, with the best survival rates obtained in Neurobasal supplemented with B27 among the tested media. After 7 days in culture, ependymal cells lose most of the cilia and therefore the mobility, while acquiring radial glial cell markers (GFAP, BLBP, GLAST). This interesting fact might indicate a reprogramming of the cell identity.
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