ArticlePLoS genetics2012
A luminal glycoprotein drives dose-dependent diameter expansion of the Drosophila melanogaster hindgut tube.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Biophysics of lumen morphogenesis.Development (Cambridge, England) · 2026Review
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- morphoHeart: A quantitative tool for integrated 3D morphometric analyses of heart and ECM during embryonic development.PLoS biology · 2025Article
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- Polarized transport of membrane and secreted proteins during lumen morphogenesis.Seminars in cell & developmental biology · 2023Review
- Morphogenetic Roles of Hydrostatic Pressure in Animal Development.Annual review of cell and developmental biology · 2022Review
- JiangShi(): a widely distributed Mucin-like protein essential for Drosophila development.G3 (Bethesda, Md.) · 2022Article
- A C. elegans Zona Pellucida domain protein functions via its ZPc domain.PLoS genetics · 2020Article
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- Extracellular matrix dynamics in tubulogenesis.Cellular signalling · 2020Review
- Physiology, Development, and Disease Modeling in theGenetics · 2020Review
- Tango1 coordinates the formation of endoplasmic reticulum/Golgi docking sites to mediate secretory granule formation.The Journal of biological chemistry · 2019Article
- QuBiT: a quantitative tool for analyzing epithelial tubes reveals unexpected patterns of organization in theDevelopment (Cambridge, England) · 2019Article
- Living tissues are more than cell clusters: The extracellular matrix as a driving force in morphogenesis.Progress in biophysics and molecular biology · 2018Review
- Tenectin recruits integrin to stabilize bouton architecture and regulate vesicle release at theeLife · 2018Article
- An Ichor-dependent apical extracellular matrix regulates seamless tube shape and integrity.PLoS genetics · 2018Article
- Distribution of Glycan Motifs at the Surface of Midgut Cells in the Cotton Leafworm (Frontiers in physiology · 2017Article
- Integrity of Narrow Epithelial Tubes in the C. elegans Excretory System Requires a Transient Luminal Matrix.PLoS genetics · 2016Article
- Developing pressures: fluid forces driving morphogenesis.Current opinion in genetics & development · 2015Review
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An important step in epithelial organ development is size maturation of the organ lumen to attain correct dimensions. Here we show that the regulated expression of Tenectin (Tnc) is critical to shape the Drosophila melanogaster hindgut tube. Tnc is a secreted protein that fills the embryonic hindgut lumen during tube diameter expansion. Inside the lumen, Tnc contributes to detectable O-Glycans and forms a dense striated matrix. Loss of tnc causes a narrow hindgut tube, while Tnc over-expression drives tube dilation in a dose-dependent manner. Cellular analyses show that luminal accumulation of Tnc causes an increase in inner and outer tube diameter, and cell flattening within the tube wall, similar to the effects of a hydrostatic pressure in other systems. When Tnc expression is induced only in cells at one side of the tube wall, Tnc fills the lumen and equally affects all cells at the lumen perimeter, arguing that Tnc acts non-cell-autonomously. Moreover, when Tnc expression is directed to a segment of a tube, its luminal accumulation is restricted to this segment and affects the surrounding cells to promote a corresponding local diameter expansion. These findings suggest that deposition of Tnc into the lumen might contribute to expansion of the lumen volume, and thereby to stretching of the tube wall. Consistent with such an idea, ectopic expression of Tnc in different developing epithelial tubes is sufficient to cause dilation, while epidermal Tnc expression has no effect on morphology. Together, the results show that epithelial tube diameter can be modelled by regulating the levels and pattern of expression of a single luminal glycoprotein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.