ArticleMolecular vision2012
Wnt3a promotes epithelial-mesenchymal transition, migration, and proliferation of lens epithelial cells.
Article in Molecular vision, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- DHRS2 regulates epithelial-mesenchymal transition in breast cancer cells.Molecular biology reports · 2026Article
- HMGB1 mediates low-dose ionizing radiation-induced Wnt/β-catenin activation in SRA01/04 cells: Mechanistic clues to early cataractogenesis.International journal of molecular medicine · 2026Article
- Anterior Capsular Contraction Syndrome in Cataract Surgery: A Review of Challenges and Solutions.Medical science monitor : international medical journal of experimental and clinical research · 2025Review
- Pax6 regulates neuronal migration and cell proliferation via interacting with Wnt3a during cortical development.Scientific reports · 2025Article
- Putative epithelial-mesenchymal transitions during salamander limb regeneration: Current perspectives and future investigations.Annals of the New York Academy of Sciences · 2024Review
- The E156K mutation in theHeliyon · 2024Article
- Wnt3a is a promising target in colorectal cancer.Medical oncology (Northwood, London, England) · 2023Review
- [Curcumin induces human lens epithelial cell apoptosis and cell cycle arrest by inhibiting Wnt/β-catenin signaling pathway].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- F-box protein FBXO16 functions as a tumor suppressor by attenuating nuclear β-catenin function.The Journal of pathology · 2019Article
- Article
- LncRNA FEZF1-AS1 Promotes TGF-Journal of ophthalmology · 2019Article
- Spatiotemporal dynamics of canonical Wnt signaling during embryonic eye development and posterior capsular opacification (PCO).Experimental eye research · 2018Article
- Acetylshikonin attenuates angiotensin II-induced proliferation and motility of human brain smooth muscle cells by inhibiting Wnt/β-catenin signaling.Human cell · 2018Article
- SH2B1 promotes epithelial-mesenchymal transition through the IRS1/β-catenin signaling axis in lung adenocarcinoma.Molecular carcinogenesis · 2018Article
- Eaf2 protects human lens epithelial cells against oxidative stress‑induced apoptosis by Wnt signaling.Molecular medicine reports · 2018Article
- EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells.BioMed research international · 2018Article
- EDIL3 depletion suppress epithelial-mesenchymal transition of lens epithelial cellsInternational journal of ophthalmology · 2018Article
- Downregulation of lncRNA OGFRP1 inhibits hepatocellular carcinoma progression by AKT/mTOR and Wnt/β-catenin signaling pathways.Cancer management and research · 2018Article
- αB-crystallin is essential for the TGF-β2-mediated epithelial to mesenchymal transition of lens epithelial cells.The Biochemical journal · 2016Article
- Long Noncoding RNA-LET Suppresses Tumor Growth and EMT in Lung Adenocarcinoma.BioMed research international · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposePosterior capsular opacification (PCO) is caused mainly by the epithelial-mesenchymal transition (EMT), proliferation, and migration of human lens epithelial (HLE) cells. wingless (Wnt) signaling has been implicated in the fibrotic process by inducing EMT and increasing the proliferation of epithelial cells. This study investigated the role of Wnt3a in PCO formation.
methodsWnt3a was overexpressed in the HLE B-3 cell line by transfected Wnt3a-pcDNA3 plasmid. The expressions of Wnt/β-catenin signaling component proteins, including β-catenin, E-cadherin, fibronectin, c-Myc, and cyclin D1, were detected by western blot analysis and immunocytofluorescence to confirm the efficiency of transfection efficiency and analyze the effects of overexpression. HLE migration ability was evaluated by transwell migration and wound healing assays, whereas HLE proliferation was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide] assay and flow cytometry.
resultsOverexpression of Wnt3a resulted in upregulated expression of β-catenin, c-Myc, and cyclin D1. Expression of the lens epithelial marker E-cadherin was down-regulated in Wnt3a-overexpressing HLE B-3 cells, whereas that of the mesenchymal marker fibronectin was upregulated. In addition, the morphology of HLE B-3 cells changed from the classic spindle shape to an irregular form. Overexpression of Wnt3a could enhance the ability of migration as determined by transwell migration and wound healing assays as well as promoted the proliferation of HLE B-3 cells by MTT assay and flow cytometry analysis.
conclusionsWnt3a can induce EMT, migration, and proliferation of HLE cells and may be a valuable therapeutic target for the prevention and treatment of PCO.
Indexed as
Identifiers
22876125PMC3413413W2119604341What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.