ArticleJournal of virology2012
Vaccinia virus virion membrane biogenesis protein A11 associates with viral membranes in a manner that requires the expression of another membrane biogenesis protein, A6.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 18 citations in OpenAlex.
- Structural analysis of conformational changes in the mpox virus A7 protein.Virologica Sinica · 2024Article
- Assembly and Evolution of Poxviruses.Advances in experimental medicine and biology · 2024Review
- The Baltimore Classification of Viruses 50 Years Later: How Does It Stand in the Light of Virus Evolution?Microbiology and molecular biology reviews : MMBR · 2021Review
- Article
- Structure of a lipid-bound viral membrane assembly protein reveals a modality for enclosing the lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Enigmatic origin of the poxvirus membrane from the endoplasmic reticulum shown by 3D imaging of vaccinia virus assembly mutants.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Vaccinia Virus A6 Is a Two-Domain Protein Requiring a Cognate N-Terminal Domain for Full Viral Membrane Assembly Activity.Journal of virology · 2017Article
- Vaccinia Virus B1 Kinase Is Required for Postreplicative Stages of the Viral Life Cycle in a BAF-Independent Manner in U2OS Cells.Journal of virology · 2015Article
- Poxvirus membrane biogenesis.Virology · 2015Review
- Structure-function analysis of vaccinia virus H7 protein reveals a novel phosphoinositide binding fold essential for poxvirus replication.Journal of virology · 2015Article
- Review
- Direct formation of vaccinia virus membranes from the endoplasmic reticulum in the absence of the newly characterized L2-interacting protein A30.5.Journal of virology · 2013Article
- Association of the vaccinia virus A11 protein with the endoplasmic reticulum and crescent precursors of immature virions.Journal of virology · 2013Article
- Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant.Journal of virology · 2013Article
- Analysis of viral membranes formed in cells infected by a vaccinia virus L2-deletion mutant suggests their origin from the endoplasmic reticulum.Journal of virology · 2013Article
- Biogenesis of the vaccinia virus membrane: genetic and ultrastructural analysis of the contributions of the A14 and A17 proteins.Journal of virology · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A group of vaccinia virus (VACV) proteins, including A11, L2, and A6, are required for biogenesis of the primary envelope of VACV, specifically, for the acquisition of viral membrane precursors. However, the interconnection among these proteins is unknown and, with the exception of L2, the connection of these proteins with membranes is also unknown. In this study, prompted by the findings that A6 coprecipitated A11 and that the cellular distribution of A11 was dramatically altered by repression of A6 expression, we studied the localization of A11 in cells by using immunofluorescence and cell fractionation analysis. A11 was found to associate with membranes and colocalize with virion membrane proteins in viral replication factories during normal VACV replication. A11 partitioned almost equally between the detergent and aqueous phases upon Triton X-114 phase separation, demonstrating an intrinsic affinity with lipids. However, in the absence of infection or VACV late protein synthesis, A11 did not associate with cellular membranes. Furthermore, when A6 expression was repressed, A11 did not colocalize with any viral membrane proteins or associate with membranes. In contrast, when virion envelope formation was blocked at a later step by repression of A14 expression or by rifampin treatment, A11 colocalized with virion membrane proteins in the factories. Altogether, our data showed that A11 associates with viral membranes during VACV replication, and this association requires A6 expression. This study provides a physical connection between A11 and viral membranes and suggests that A6 regulates A11 membrane association.
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