ArticleRNA (New York, N.Y.)2012
MicroRNAs in Amoebozoa: deep sequencing of the small RNA population in the social amoeba Dictyostelium discoideum reveals developmentally regulated microRNAs.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Chromosome-level genome assembly and annotation of the social amoeba Dictyostelium firmibasis.Scientific data · 2024Article
- Evolution of microRNAs in Amoebozoa and implications for the origin of multicellularity.Nucleic acids research · 2024Article
- Small RNAs in Cnidaria: A review.Evolutionary applications · 2023Article
- Time-resolved small RNA transcriptomics of the ichthyosporeanF1000Research · 2023Article
- In Silico Identification and Characterization of circRNAs as Potential Virulence-Related miRNA/siRNA Sponges fromNon-coding RNA · 2022Article
- Fractional 2'-O-methylation in the ribosomal RNA of Dictyostelium discoideum supports ribosome heterogeneity in Amoebozoa.Scientific reports · 2022Article
- Abundantly expressed class of noncoding RNAs conserved through the multicellular evolution of dictyostelid social amoebas.Genome research · 2021Article
- DIRS retrotransposons amplify via linear, single-stranded cDNA intermediates.Nucleic acids research · 2020Article
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model.BMC genomics · 2019Article
- Too Many False Targets for MicroRNAs: Challenges and Pitfalls in Prediction of miRNA Targets and Their Gene Ontology in Model and Non-model Organisms.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Review
- Genome-wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips,Ecology and evolution · 2018Article
- Metazoan MicroRNAs.Cell · 2018Review
- Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum.RNA biology · 2018Article
- miRNAs in the alga Chlamydomonas reinhardtii are not phylogenetically conserved and play a limited role in responses to nutrient deprivation.Scientific reports · 2017Article
- Towards a Consistent, Quantitative Evaluation of MicroRNA Evolution.Journal of integrative bioinformatics · 2017Article
- Rapid Evolution of microRNA Loci in the Brown Algae.Genome biology and evolution · 2017Article
- The evolutionary origin of plant and animal microRNAs.Nature ecology & evolution · 2017Article
- The Long Noncoding RNA Transcriptome ofG3 (Bethesda, Md.) · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA interference machinery has served as a guardian of eukaryotic genomes since the divergence from prokaryotes. Although the basic components have a shared origin, silencing pathways directed by small RNAs have evolved in diverse directions in different eukaryotic lineages. Micro (mi)RNAs regulate protein-coding genes and play vital roles in plants and animals, but less is known about their functions in other organisms. Here, we report, for the first time, deep sequencing of small RNAs from the social amoeba Dictyostelium discoideum. RNA from growing single-cell amoebae as well as from two multicellular developmental stages was sequenced. Computational analyses combined with experimental data reveal the expression of miRNAs, several of them exhibiting distinct expression patterns during development. To our knowledge, this is the first report of miRNAs in the Amoebozoa supergroup. We also show that overexpressed miRNA precursors generate miRNAs and, in most cases, miRNA* sequences, whose biogenesis is dependent on the Dicer-like protein DrnB, further supporting the presence of miRNAs in D. discoideum. In addition, we find miRNAs processed from hairpin structures originating from an intron as well as from a class of repetitive elements. We believe that these repetitive elements are sources for newly evolved miRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.