ArticleHistochemistry and cell biology2012
Postfixation detergent treatment liberates the membrane modelling protein Pex11β from peroxisomal membranes.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Immunolabeling for Detection of Endogenous and Overexpressed Peroxisomal Proteins in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- PEX11β and FIS1 cooperate in peroxisome division independently of mitochondrial fission factor.Journal of cell science · 2022Article
- Determinants of Peroxisome Membrane Dynamics.Frontiers in physiology · 2022Review
- A Functional SMAD2/3 Binding Site in theFrontiers in cell and developmental biology · 2020Article
- Variant analysis of PEX11B gene from a family with peroxisome biogenesis disorder 14B by whole exome sequencing.Molecular genetics & genomic medicine · 2020Article
- Novel PEX11B Mutations Extend the Peroxisome Biogenesis Disorder 14B Phenotypic Spectrum and Underscore Congenital Cataract as an Early Feature.Investigative ophthalmology & visual science · 2017Article
- Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins.The Journal of biological chemistry · 2016Article
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pex11 proteins are involved in membrane remodelling processes of peroxisomes, and are key components of peroxisomal division and proliferation. In mammals, three Pex11 isoforms, Pex11α, Pex11β, and Pex11γ exist. Here we demonstrate that Pex11β, but not Pex11α or Pex11γ, is almost exclusively extracted from peroxisomal membranes of paraformaldehyde-fixed cells by permeabilisation with the non-ionic detergent Triton X-100. This results in diminished detection of Myc-Pex11β in immunofluorescence preparations and appearance of the protein in the Triton X-100 extract. To our knowledge, Pex11β is the first peroxisomal membrane protein showing such a peculiar behaviour. Loss of Pex11β can be avoided by permeabilisation with digitonin, the addition of glutaraldehyde to the fixative, or the expression of a Pex11 fusion protein with a larger protein tag (e.g. YFP). Our observations further point to different functions and biochemical properties of the Pex11 isoforms within the peroxisomal membrane and during peroxisome proliferation.
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