ArticleChannels (Austin, Tex.)
Characterization of three novel mechanosensitive channel activities in Escherichia coli.
Article in Channels (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- The diverse gating mechanisms of bacterial MscS-like ion channels.Biochemical Society transactions · 2026Review
- The MscS channel frombioRxiv : the preprint server for biology · 2026Article
- Cytotoxicity mechanism of heterologous expression of phospholipase D inMicrobiology spectrum · 2026Article
- The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.Nature communications · 2026Article
- MscM uses a novel gating mechanism for bacterial mechanosensitive channels.bioRxiv : the preprint server for biology · 2026Article
- Article
- Cell Adhesion and Biofilm Development via Force-Sensitive Mechanisms: A Perspective.ACS biomaterials science & engineering · 2026Review
- Mechanosensitive channel engineering: A study on the mixing and matching of YnaI and MscS sensor paddles and pores.Nature communications · 2025Article
- The diverse phenotypic and mutational landscape induced by fluoroquinolone treatment.mSystems · 2025Article
- Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI.Nature communications · 2025Article
- Lipid-mediated gating of a miniature mechanosensitive MscS channel from Trypanosoma cruzi.Nature communications · 2025Article
- InmicroPublication biology · 2025Article
- Effects ofMicroorganisms · 2024Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- Catabolism and interactions of syntrophic propionate- and acetate oxidizing microorganisms under mesophilic, high-ammonia conditions.Frontiers in microbiology · 2024Article
- Article
- Origin, evolution and diversification of plant mechanosensitive channel of small conductance-like (MSL) proteins.BMC plant biology · 2023Article
- Article
- The Effects of Airflow on the Mechanosensitive Channels ofMicroorganisms · 2023Article
22 more citing papers are in PubMed but not listed here.
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8 authors.
Funding
Abstract
Mechanosensitive channels sense elevated membrane tension that arises from rapid water influx occurring when cells move from high to low osmolarity environments (hypoosmotic shock). These non-specific channels in the cytoplasmic membrane release osmotically-active solutes and ions. The two major mechanosensitive channels in Escherichia coli are MscL and MscS. Deletion of both proteins severely compromises survival of hypoosmotic shock. However, like many bacteria, E. coli cells possess other MscS-type genes (kefA, ybdG, ybiO, yjeP and ynaI). Two homologs, MscK (kefA) and YbdG, have been characterized as mechanosensitive channels that play minor roles in maintaining cell integrity. Additional channel openings are occasionally observed in patches derived from mutants lacking MscS, MscK and MscL. Due to their rare occurrence, little is known about these extra pressure-induced currents or their genetic origins. Here we complete the identification of the remaining E. coli mechanosensitive channels YnaI, YbiO and YjeP. The latter is the major component of the previously described MscM activity (~300 pS), while YnaI (~100 pS) and YbiO (~1000 pS) were previously unknown. Expression of native YbiO is NaCl-specific and RpoS-dependent. A Δ7 strain was created with all seven E. coli mechanosensitive channel genes deleted. High level expression of YnaI, YbiO or YjeP proteins from a multicopy plasmid in the Δ7 strain (MJFGH) leads to substantial protection against hypoosmotic shock. Purified homologs exhibit high molecular masses that are consistent with heptameric assemblies. This work reveals novel mechanosensitive channels and discusses the regulation of their expression in the context of possible additional functions.
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