ArticleJournal of the American Chemical Society2012
Raising the speed limit for β-hairpin formation.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Highly parallelizable path sampling with minimal rejections using asynchronous replica exchange and infinite swaps.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Molecular Free Energies, Rates, and Mechanisms from Data-Efficient Path Sampling Simulations.Journal of chemical theory and computation · 2023Article
- Visualization of Self-Assembly and Hydration of a β-Hairpin through Integrated Small and Wide-Angle Neutron Scattering.Biomacromolecules · 2023Article
- Granger Causality Analysis of Chignolin Folding.Journal of chemical theory and computation · 2022Article
- ff19SB: Amino-Acid-Specific Protein Backbone Parameters Trained against Quantum Mechanics Energy Surfaces in Solution.Journal of chemical theory and computation · 2020Article
- A quantitative connection of experimental and simulated folding landscapes by vibrational spectroscopy.Chemical science · 2018Article
- Modeling the mechanism of CLN025 beta-hairpin formation.The Journal of chemical physics · 2017Article
- Building a More Predictive Protein Force Field: A Systematic and Reproducible Route to AMBER-FB15.The journal of physical chemistry. B · 2017Article
- Article
- When fast is better: protein folding fundamentals and mechanisms from ultrafast approaches.The Biochemical journal · 2016Review
- Influence of Glu/Arg, Asp/Arg, and Glu/Lys Salt Bridges on α-Helical Stability and Folding Kinetics.Biophysical journal · 2016Article
- ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB.Journal of chemical theory and computation · 2015Article
- Direct evidence of catalytic heterogeneity in lactate dehydrogenase by temperature jump infrared spectroscopy.The journal of physical chemistry. B · 2014Article
- Fast protein folding kinetics.Quarterly reviews of biophysics · 2014Review
- How quickly can a β-hairpin fold from its transition state?The journal of physical chemistry. B · 2014Article
- Tracing primordial protein evolution through structurally guided stepwise segment elongation.The Journal of biological chemistry · 2014Article
- Dynamics of an ultrafast folding subdomain in the context of a larger protein fold.Journal of the American Chemical Society · 2013Article
- Mutational effects on the folding dynamics of a minimized hairpin.Biochemistry · 2013Article
- Microsecond folding experiments and simulations: a match is made.Physical chemistry chemical physics : PCCP · 2013Article
- Early turn formation and chain collapse drive fast folding of the major cold shock protein CspA of Escherichia coli.Biochemistry · 2012Article
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Authors and funding
4 authors.
Funding
Abstract
Understanding the folding of the β-hairpin is a crucial step in studying how β-rich proteins fold. We have studied CLN025, an optimized ten residue synthetic peptide, which adopts a compact, well-structured β-hairpin conformation. Formation of the component β-sheet and β-turn structures of CLN025 was probed independently using a combination of equilibrium Fourier transform infrared spectroscopy and laser-induced temperature jump coupled with time-resolved infrared and fluorescence spectroscopies. We find that CLN025 is an ultrafast folder due to its small free energy barrier to folding and that it exceeds the predicted speed limit for β-hairpin formation by an order of magnitude. We also find that the folding mechanism cannot be described by a simple two-state model, but rather is a heterogeneous process involving two independent parallel processes. Formation of stabilizing cross-strand hydrophobic interactions and turn alignment occur competitively, with relaxation lifetimes of 82 ± 10 and 124 ± 10 ns, respectively, at the highest probed temperature. The ultrafast and heterogeneous folding kinetics observed for CLN025 provide evidence for folding on a nearly barrierless free energy landscape, and recalibrate the speed limit for the formation of a β-hairpin.
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