ArticleStem cells and development2012
The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process.
Article in Stem cells and development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 83 citations in OpenAlex.
- Post-translational MYC stabilization in association with IGF-1 signaling during corneal epithelial repair.Experimental eye research · 2026Article
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- Silencing Ubiquitin-Specific Peptidase 10 Alleviates Persistent Corneal Epithelial Defect in Mice.The American journal of pathology · 2026Article
- Repair of Corneal Epithelial Defects.Progress in molecular and subcellular biology · 2026Review
- Review
- Oxygen uptake at the ocular surface in diabetic animals is impaired in response to central corneal injury.Experimental eye research · 2025Article
- Navigating the path to corneal healing success and challenges: a comprehensive overview.Eye (London, England) · 2025Review
- Review
- The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells.Frontiers in cell and developmental biology · 2025Article
- Topical insulin to treat a conjunctival defect after glaucoma surgery: A case report.American journal of ophthalmology case reports · 2024Article
- Review
- Focus on seed cells: stem cells in 3D bioprinting of corneal grafts.Frontiers in bioengineering and biotechnology · 2024Review
- Elucidating the mechanism of corneal epithelial cell repair: unraveling the impact of growth factors.Frontiers in medicine · 2024Review
- Review
- Enhanced adipose-derived stem cells with IGF-1-modified mRNA promote wound healing following corneal injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Safety, Tolerability, and Serum/Tear Pharmacokinetics of Human Recombinant Epidermal Growth Factor Eyedrops in Healthy Subjects.Pharmaceuticals (Basel, Switzerland) · 2022Article
- WNT16B enhances the proliferation and self-renewal of limbal epithelial cells via CXCR4/MEK/ERK signaling.Stem cell reports · 2022Article
- Article
- Equine Umbilical Cord Serum Composition and Its Healing Effects in Equine Corneal Ulceration.Frontiers in veterinary science · 2022Article
- Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet.Nutrients · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Limbal stem cells (LSC), which reside in the basal layer of the limbus, are thought to be responsible for corneal epithelial healing after injury. When the cornea is damaged, LSC start to proliferate, differentiate, and migrate to the site of injury. To characterize the signaling molecules ensuring communication between the cornea and LSC, we established a mouse model of mechanical corneal damage. The central cornea or limbal tissue was excised at different time intervals after injury, and the expression of genes in the explants was determined. It was observed that a number of genes for growth and differentiation factors were significantly upregulated in the cornea rapidly after injury. The ability of these factors to regulate the differentiation and proliferation of limbal cells was tested. It was found that the insulin-like growth factor-I (IGF-I), which is rapidly overexpressed after injury, enhances the expression of IGF receptor in limbal cells and induces the differentiation of LSC into cells expressing the corneal cell marker, cytokeratin K12, without any effect on limbal cell proliferation. In contrast, the epidermal growth factor (EGF) and fibroblast growth factor-β (FGF-β), which are also produced by the damaged corneal epithelium, supported limbal cell proliferation without any effect on their differentiation. Other factors did not affect limbal cell differentiation or proliferation. Thus, IGF-I was identified as the main factor stimulating the expression of IGF receptors in limbal cells and inducing the differentiation of LSC into cells expressing corneal epithelial cell markers. The proliferation of these cells was supported by EGF and FGF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.