ArticleProceedings of the National Academy of Sciences of the United States of America2012
Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 124 citations in OpenAlex.
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- Whole-cell modeling predicts alternative proteome allocation strategies in the archaeon Methanococcus maripaludis.Scientific reports · 2026Article
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- Functionally redundant formate dehydrogenases enable formate-dependent growth in Methanococcus maripaludis.The Journal of biological chemistry · 2024Article
- Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus.Nature microbiology · 2023Article
- Evolving understanding of rumen methanogen ecophysiology.Frontiers in microbiology · 2023Review
- Serpentinization as the source of energy, electrons, organics, catalysts, nutrients and pH gradients for the origin of LUCA and life.Frontiers in microbiology · 2023Review
- Formate and hydrogen in hydrothermal vents and their use by extremely thermophilic methanogens and heterotrophs.Frontiers in microbiology · 2023Review
- Interspecies Formate Exchange Drives Syntrophic Growth ofApplied and environmental microbiology · 2022Article
- Genomic remnants of ancestral methanogenesis and hydrogenotrophy in Archaea drive anaerobic carbon cycling.Science advances · 2022Article
- Growth rate-dependent coordination of catabolism and anabolism in the archaeon Methanococcus maripaludis under phosphate limitation.The ISME journal · 2022Article
- Proteomic Analysis of Methanococcus voltae Grown in the Presence of Mineral and Nonmineral Sources of Iron and Sulfur.Microbiology spectrum · 2022Article
2 more citing papers are in PubMed but not listed here.
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6 authors at 3 institutions in 2 countries.
Funding
Abstract
Despite decades of study, electron flow and energy conservation in methanogenic Archaea are still not thoroughly understood. For methanogens without cytochromes, flavin-based electron bifurcation has been proposed as an essential energy-conserving mechanism that couples exergonic and endergonic reactions of methanogenesis. However, an alternative hypothesis posits that the energy-converting hydrogenase Eha provides a chemiosmosis-driven electron input to the endergonic reaction. In vivo evidence for both hypotheses is incomplete. By genetically eliminating all nonessential pathways of H(2) metabolism in the model methanogen Methanococcus maripaludis and using formate as an additional electron donor, we isolate electron flow for methanogenesis from flux through Eha. We find that Eha does not function stoichiometrically for methanogenesis, implying that electron bifurcation must operate in vivo. We show that Eha is nevertheless essential, and a substoichiometric requirement for H(2) suggests that its role is anaplerotic. Indeed, H(2) via Eha stimulates methanogenesis from formate when intermediates are not otherwise replenished. These results fit the model for electron bifurcation, which renders the methanogenic pathway cyclic, and as such requires the replenishment of intermediates. Defining a role for Eha and verifying electron bifurcation provide a complete model of methanogenesis where all necessary electron inputs are accounted for.
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