ArticleHistochemistry and cell biology2012
On the ultrastructural organization of Trypanosoma cruzi using cryopreparation methods and electron tomography.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Cell Death and Unprecedent Cytoskeletal Changes inChemical & biomedical imaging · 2026Article
- Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy.Journal of microscopy · 2026Article
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- The subcellular architecture ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi.Journal of cell science · 2025Article
- Outlining the structure of cAMP compartments in Trypanosoma cruzi.Trends in parasitology · 2025Review
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- Quantitative assessment of the nanoanatomy of the contractile vacuole complex inLife science alliance · 2024Article
- Structure and dynamics of the contractile vacuole complex in Tetrahymena thermophila.Journal of cell science · 2023Article
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- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- Protozoan phagotrophy from predators to parasites: An overview of the enigmatic cytostome-cytopharynx complex of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- Animal Models ofPathogens (Basel, Switzerland) · 2022Review
- Electron Microscopy Techniques Applied to Symbiont-Harboring Trypanosomatids: The Association of the Bacterium with Host Organelles.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- Dependency relationships between IFT-dependent flagellum elongation and cell morphogenesis inOpen biology · 2018Article
- A comparative molecular and 3-dimensional structural investigation into cross-continental and novel avian Trypanosoma spp. in Australia.Parasites & vectors · 2017Article
- Article
- Virtual Reconstruction and Three-Dimensional Printing of Blood Cells as a Tool in Cell Biology Education.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structural organization of Trypanosoma cruzi has been intensely investigated by different microscopy techniques. At the electron microscopy level, bi-dimensional analysis of thin sections of chemically fixed cells has been one of the most commonly used techniques, despite the known potential of generating artifacts during chemical fixation and the subsequent steps of sample preparation. In contrast, more sophisticated and elaborate techniques, such as cryofixation followed by freeze substitution that are known to preserve the samples in a more close-to-native state, have not been widely applied to T. cruzi. In addition, the 3D characterization of such cells has been carried out mostly using 3D reconstruction from serial sections, currently considered a low resolution technique when compared to electron tomography (ET). In this work, we re-visited the 3D ultrastructure of T. cruzi using a combination of two approaches: (1) analysis of both conventionally processed and cryofixed and freeze substituted cells and (2) 3D reconstruction of large volumes by serial electron tomography. The analysis of high-pressure frozen and freeze substituted parasites showed novel characteristics in a number of intracellular structures, both in their structure and content. Organelles generally showed a smooth and regular morphology in some cases presenting a characteristic electron dense content. Ribosomes and new microtubule sets showed an unexpected localization in the cell body. The improved preservation and imaging in 3D of T. cruzi cells using cryopreparation techniques has revealed some novel aspects of the ultrastructural organization of this parasite.
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