ArticlePloS one2012
De novo sequencing and transcriptome analysis of the central nervous system of mollusc Lymnaea stagnalis by deep RNA sequencing.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
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- A multiscale computational framework for the development of spines in molluscan shells.PLoS computational biology · 2024Article
- Yolk proteins of the schistosomiasis vector snail Biomphalaria glabrata revealed by multi-omics analysis.Scientific reports · 2024Article
- Lack of membrane sex steroid receptors for mediating rapid endocrine responses in molluscan nervous systems.Frontiers in endocrinology · 2024Article
- FOXO inBiology · 2023Article
- Transcriptome Sequencing ofPlants (Basel, Switzerland) · 2023Article
- Identification of Putative Molecules for Adiponectin and Adiponectin Receptor and Their Roles in Learning and Memory inBiology · 2023Article
- Changes in protein phosphorylation by insulin administration in the central nervous system of the gastropod molluskBiophysics and physicobiology · 2023Article
- Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Identification and characterization of the kynurenine pathway in the pond snail Lymnaea stagnalis.Scientific reports · 2022Article
- Epigenetic machinery is functionally conserved in cephalopods.BMC biology · 2022Article
- Context-Dependent Role of miR-124 in Retinoic Acid-Induced Growth Cone Attraction of Regenerating Motorneurons.Cellular and molecular neurobiology · 2022Article
- Transcriptome analysis provides genome annotation and expression profiles in the central nervous system of Lymnaea stagnalis at different ages.BMC genomics · 2021Article
- Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis.BMC genomics · 2021Article
- Identification and classification of innexin gene transcripts in the central nervous system of the terrestrial slug Limax valentianus.PloS one · 2021Article
- Neuropeptide Localization inFrontiers in molecular neuroscience · 2021Review
- The pond snail Lymnaea stagnalis.EvoDevo · 2020Review
- Early Metazoan Origin and Multiple Losses of a Novel Clade of RIM Presynaptic Calcium Channel Scaffolding Protein Homologs.Genome biology and evolution · 2020Article
- Functional shell matrix proteins tentatively identified by asymmetric snail shell morphology.Scientific reports · 2020Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pond snail Lymnaea stagnalis is among several mollusc species that have been well investigated due to the simplicity of their nervous systems and large identifiable neurons. Nonetheless, despite the continued attention given to the physiological characteristics of its nervous system, the genetic information of the Lymnaea central nervous system (CNS) has not yet been fully explored. The absence of genetic information is a large disadvantage for transcriptome sequencing because it makes transcriptome assembly difficult. We here performed transcriptome sequencing for Lymnaea CNS using an Illumina Genome Analyzer IIx platform and obtained 81.9 M of 100 base pair (bp) single end reads. For de novo assembly, five programs were used: ABySS, Velvet, OASES, Trinity and Rnnotator. Based on a comparison of the assemblies, we chose the Rnnotator dataset for the following blast searches and gene ontology analyses. The present dataset, 116,355 contigs of Lymnaea transcriptome shotgun assembly (TSA), contained longer sequences and was much larger compared to the previously reported Lymnaea expression sequence tag (EST) established by classical Sanger sequencing. The TSA sequences were subjected to blast analyses against several protein databases and Aplysia EST data. The results demonstrated that about 20,000 sequences had significant similarity to the reported sequences using a cutoff value of 1e-6, and showed the lack of molluscan sequences in the public databases. The richness of the present TSA data allowed us to identify a large number of new transcripts in Lymnaea and molluscan species.
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