Trial reportPloS one2012
Quantitative proteomic analysis of the effect of fluoride on the acquired enamel pellicle.
Trial report in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 79 citations in OpenAlex.
- Effect of a sodium fluoride rinse on the composition of early oral biofilms formed in situ on enamel and dentine.Clinical oral investigations · 2026Trial
- Trial
- Evaluation of Microleakage Beneath Orthodontic Molar Bands Cemented With Resin-Modified Glass Ionomer Using Two Enamel Deproteinization Agents.International journal of dentistry · 2026Article
- Global Salivary Proteomic Profiling of Individuals with the Co-Occurrence of Type 2 Diabetes Mellitus, Dyslipidemia, and Periodontitis.International journal of molecular sciences · 2025Article
- In Vitro Assessment of Salivary Pellicle Disruption and Biofilm Removal on Titanium: Exploring the Role of Surface Hydrophobicity in Chemical Disinfection.Clinical and experimental dental research · 2025Article
- Acquired pellicle engineering: a fascinating approach to prevent demineralization.Journal of applied oral science : revista FOB · 2025Review
- Proanthocyanidin associated to vitamin E or palm oil on initial enamel erosion: in situ and in vitro study.Brazilian oral research · 2025Article
- Effect of pellicle modification with polyphenol-rich solutions on enamel erosion and abrasion.Brazilian oral research · 2025Article
- Article
- Caries Management-The Role of Surface Interactions in De- and Remineralization-Processes.Journal of clinical medicine · 2022Review
- Synergistic effect between plant extracts and fluoride to protect against enamel erosion: An in vitro study.PloS one · 2022Article
- Efficacy of propolis in remineralising artificially induced demineralisation of human enamel - An in-vitro study.Journal of Taibah University Medical Sciences · 2021Article
- Evaluating protein binding specificity of titanium surfaces through mass spectrometry-based proteomics.Clinical oral investigations · 2021Article
- Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.PloS one · 2021Article
- Salivary proteomic profile of dogs with and without dental calculus.BMC veterinary research · 2020Article
- Analysis of Cytoplasmic and Secreted Proteins ofMicroorganisms · 2020Article
- Article
- Article
- Optimizing the formation of the acquired enamel pellicle in vitro for proteomic analysis.Journal of applied oral science : revista FOB · 2020Article
- Gene expression and protein synthesis of esterase from Streptococcus mutans are affected by biodegradation by-product from methacrylate resin composites and adhesives.Acta biomaterialia · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
Abstract
The acquired enamel pellicle (AEP) is a thin film formed by the selective adsorption of salivary proteins onto the enamel surface of teeth. The AEP forms a critical interface between the mineral phase of teeth (hydroxyapatite) and the oral microbial biofilm. This biofilm is the key feature responsible for the development of dental caries. Fluoride on enamel surface is well known to reduce caries by reducing the solubility of enamel to acid. Information on the effects of fluoride on AEP formation is limited. This study aimed to investigate the effects of fluoride treatment on hydroxyapatite on the subsequent formation of AEP. In addition, this study pioneered the use of label-free quantitative proteomics to better understand the composition of AEP proteins. Hydroxyapatite discs were randomly divided in 4 groups (n = 10 per group). Each disc was exposed to distilled water (control) or sodium fluoride solution (1, 2 or 5%) for 2 hours. Discs were then washed and immersed in human saliva for an additional 2 hours. AEP from each disc was collected and subjected to liquid chromatography electrospray ionization mass spectrometry for protein identification, characterization and quantification. A total of 45 proteins were present in all four groups, 12 proteins were exclusively present in the control group and another 19 proteins were only present in the discs treated with 5% sodium fluoride. Relative proteomic quantification was carried out for the 45 proteins observed in all four groups. Notably, the concentration of important salivary proteins, such as statherin and histatin 1, decrease with increasing levels of fluoride. It suggests that these proteins are repulsed when hydroxyapatite surface is coated with fluoride. Our data demonstrated that treatment of hydroxyapatite with fluoride (at high concentration) qualitatively and quantitatively modulates AEP formation, effects which in turn will likely impact the formation of oral biofilms.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.