ArticlePloS one2012
Resistance to BmNPV via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, Bombyx mori.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Article
- A Study on the Effect of Energy on the Development of Silkworm Embryos Using an Estrogen-Related Receptor.International journal of molecular sciences · 2023Article
- A Protein Asteroid with PIN Domain in SilkwormInsects · 2023Article
- SericinInsects · 2023Article
- Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus AffectingInternational journal of molecular sciences · 2022Article
- Transgenic Silkworms Overexpressing Relish and Expressing Drosomycin Confer Enhanced Immunity to Multiple Pathogens.Molecular biotechnology · 2022Article
- The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm,International journal of molecular sciences · 2021Article
- Insights Into the Antiviral Pathways of the SilkwormFrontiers in immunology · 2021Review
- Advances in the Arms Race Between Silkworm and Baculovirus.Frontiers in immunology · 2021Review
- Toxicological evaluation of transgenic silkworms.Toxicology research · 2020Article
- PI3K-Akt pathway mediated antiviral mechanism in silkwormVirusdisease · 2020Article
- Spliceosomal Protein GeneInternational journal of molecular sciences · 2020Article
- Optimization of a 2A self-cleaving peptide-based multigene expression system for efficient expression of upstream and downstream genes in silkworm.Molecular genetics and genomics : MGG · 2019Article
- Screening of PI3K-Akt-targeting Drugs for Silkworm againstMolecules (Basel, Switzerland) · 2019Article
- Distinct Functions ofFrontiers in immunology · 2019Article
- Transcriptome analysis of the response of silkworm to drastic changes in ambient temperature.Applied microbiology and biotechnology · 2018Article
- Exogenous gene can be expressed by a recombinant Bombyx mori cypovirus.Applied microbiology and biotechnology · 2018Article
- Construction and characterization of a synthetic Baculovirus-inducible 39K promoter.Journal of biological engineering · 2018Article
- Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System.Frontiers in microbiology · 2018Article
- Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus.Journal of virology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hycu-ep32 gene of Hyphantria cunea NPV can inhibit Bombyx mori nucleopolyhedrovirus (BmNPV) multiplication in co-infected cells, but it is not known whether the overexpression of the hycu-ep32 gene has an antiviral effect in the silkworm, Bombyx mori. Thus, we constructed four transgenic vectors, which were under the control of the 39 K promoter of BmNPV (39 KP), Bombyx mori A4 promoter (A4P), hr3 enhancer of BmNPV combined with 39 KP, and hr3 combined with A4P. Transgenic lines were created via embryo microinjection using practical diapause silkworm. qPCR revealed that the expression level of hycu-ep32 could be induced effectively after BmNPV infection in transgenic lines where hycu-ep32 was controlled by hr3 combined with 39 KP (i.e., HEKG). After oral inoculation of BmNPV with 3 × 10(5) occlusion bodies per third instar, the mortality with HEKG-B was approximately 30% lower compared with the non-transgenic line. The economic characteristics of the transgenic lines remained unchanged. These results suggest that overexpression of an exogenous antiviral gene controlled by an inducible promoter and enhancer is a feasible method for breeding silkworms with a high antiviral capacity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.