ArticlePloS one2012
Magnetic forces and DNA mechanics in multiplexed magnetic tweezers.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 68 citations in OpenAlex.
- MTERF1 polar roadblock mechanism revealed using high-throughput magnetic tweezers and accurate single-bead force calibration.Nucleic acids research · 2026Article
- Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.Annual review of biophysics · 2026Review
- DNA twist at high alkali ion concentrations: evidence against C-form DNA in solution.Nucleic acids research · 2026Article
- A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments.Nature protocols · 2025Review
- Single-molecule force stability of the SARS-CoV-2-ACE2 interface in variants-of-concern.Nature nanotechnology · 2024Article
- Combining DNA scaffolds and acoustic force spectroscopy to characterize individual protein bonds.Biophysical journal · 2023Article
- Article
- Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Twist-diameter coupling drives DNA twist changes with salt and temperature.Science advances · 2022Article
- High-throughput mechanobiology: Force modulation of ensemble biochemical and cell-based assays.Biophysical journal · 2021Article
- Wi-Fi Live-Streaming Centrifuge Force Microscope for Benchtop Single-Molecule Experiments.Biophysical journal · 2020Article
- Multiplexed Nanometric 3D Tracking of Microbeads Using an FFT-Phasor Algorithm.Biophysical journal · 2020Article
- Review
- Multiplexed protein force spectroscopy reveals equilibrium protein folding dynamics and the low-force response of von Willebrand factor.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Submicrometer elasticity of double-stranded DNA revealed by precision force-extension measurements with magnetic tweezers.Science advances · 2019Article
- The mechanism of DNA unwinding by the eukaryotic replicative helicase.Nature communications · 2019Article
- The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers.Biophysical journal · 2019Article
- Correction-free force calibration for magnetic tweezers experiments.Scientific reports · 2018Article
- Long repeating (TTAGGG)The Journal of biological chemistry · 2018Article
- Real-time detection of condensin-driven DNA compaction reveals a multistep binding mechanism.The EMBO journal · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnetic tweezers (MT) are a powerful tool for the study of DNA-enzyme interactions. Both the magnet-based manipulation and the camera-based detection used in MT are well suited for multiplexed measurements. Here, we systematically address challenges related to scaling of multiplexed magnetic tweezers (MMT) towards high levels of parallelization where large numbers of molecules (say 10(3)) are addressed in the same amount of time required by a single-molecule measurement. We apply offline analysis of recorded images and show that this approach provides a scalable solution for parallel tracking of the xyz-positions of many beads simultaneously. We employ a large field-of-view imaging system to address many DNA-bead tethers in parallel. We model the 3D magnetic field generated by the magnets and derive the magnetic force experienced by DNA-bead tethers across the large field of view from first principles. We furthermore experimentally demonstrate that a DNA-bead tether subject to a rotating magnetic field describes a bicircular, Limaçon rotation pattern and that an analysis of this pattern simultaneously yields information about the force angle and the position of attachment of the DNA on the bead. Finally, we apply MMT in the high-throughput investigation of the distribution of the induced magnetic moment, the position of attachment of DNA on the beads, and DNA flexibility. The methods described herein pave the way to kilo-molecule level magnetic tweezers experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.