ArticleProceedings of the National Academy of Sciences of the United States of America2012
Reprogramming IgH isotype-switched B cells to functional-grade induced pluripotent stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Article
- Knockdown H19 Accelerated iPSCs Reprogramming through Epigenetic Modifications and Mesenchymal-to-Epithelial Transition.Biomolecules · 2024Article
- Targeting EZH1/2 induces cell cycle arrest and inhibits cell proliferation through reactivation of p57Cancer biology & medicine · 2019Article
- IgE Glycosylation in Health and Disease.Current topics in microbiology and immunology · 2019Review
- Insights From Analysis of Human Antigen-Specific Memory B Cell Repertoires.Frontiers in immunology · 2018Review
- IgH isotype-specific B cell receptor expression influences B cell fate.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- IgG1 memory B cells keep the memory of IgE responses.Nature communications · 2017Article
- Epigenetic regulation of the lncRNA MEG3 and its target c-MET in pancreatic neuroendocrine tumors.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Detection of true IgE-expressing mouse B lineage cells.Journal of visualized experiments : JoVE · 2014Article
- DLK1-DIO3 imprinted cluster in induced pluripotency: landscape in the mist.Cellular and molecular life sciences : CMLS · 2014Review
- Lymphocyte-based model systems for allergy research: a historic overview.International archives of allergy and immunology · 2014Review
- The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response.The Journal of experimental medicine · 2013Article
- Induced pluripotency of human prostatic epithelial cells.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Induced pluripotent stem cells (iPSCs) can be formed from somatic cells by a defined set of genetic factors; however, aberrant epigenetic silencing of the imprinted Dlk1-Dio3 gene cluster often hinders their developmental potency and ability to contribute to high-grade chimerism in mice. Here, we describe an approach that allows splenic B cells activated to undergo Ig heavy-chain (IgH) class-switch recombination (CSR) to be reprogrammed into iPSCs that contribute to high-grade chimerism in mice. Treatment of naïve splenic B cells in culture with anti-CD40 plus IL-4 induces IgH CSR from IgM to IgG1 and IgE. CSR leads to irreversible IgH locus deletions wherein the IgM-producing Cμ exons are permanently excised from the B-cell genome. We find that anti-CD40 plus IL-4-activated B cells produce iPSCs that are uniformly hypermethylated in the imprinted Dlk1-Dio3 gene cluster and fail to produce chimerism in mice. However, treatment of activated B cells with the methyltransferase inhibitor 5-aza-2'-deoxycytidine before and at early stages of reprogramming attenuates hypermethylation of the Dlk1-Dio3 locus in resultant iPSCs and enables them to form high-grade chimerism in mice. These conditions allowed us to produce chimeric mice in which all mature B cells were derived entirely from IgG1-expressing B-cell-derived iPSCs. We conclude that culture conditions of activated B cells before and at early stages of reprogramming influence the developmental potency of resultant iPSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.