ArticleProceedings of the National Academy of Sciences of the United States of America2012
Inducible genetic system for the axolotl.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Streptozotocin induced hyperglycemia in the axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.Methods (San Diego, Calif.) · 2025Article
- Adeno-associated viruses for efficient gene expression in the axolotl nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Baculovirus Production and Infection in Axolotls.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The use of transgenics in the laboratory axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
- The Axolotl's journey to the modern molecular era.Current topics in developmental biology · 2022Article
- The Diverse Manifestations of Regeneration and Why We Need to Study Them.Cold Spring Harbor perspectives in biology · 2021Article
- The InducibleFrontiers in genetics · 2021Article
- von Willebrand factor D and EGF domains is an evolutionarily conserved and required feature of blastemas capable of multitissue appendage regeneration.Evolution & development · 2020Article
- Advancements to the Axolotl Model for Regeneration and Aging.Gerontology · 2020Review
- Precise control of ion channel and gap junction expression is required for patterning of the regenerating axolotl limb.The International journal of developmental biology · 2020Article
- Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum).Nature protocols · 2018Article
- Advances in Decoding Axolotl Limb Regeneration.Trends in genetics : TIG · 2017Review
- Non-model model organisms.BMC biology · 2017Article
- Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl.Developmental biology · 2017Article
- Identification of regenerative roadblocks via repeat deployment of limb regeneration in axolotls.NPJ Regenerative medicine · 2017Article
- Evaluation of the anesthetic effects of MS222 in the adult Mexican axolotl (Veterinary medicine (Auckland, N.Z.) · 2016Article
- Budgett's frog (Lepidobatrachus laevis): A new amphibian embryo for developmental biology.Developmental biology · 2015Article
- TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl.Regeneration (Oxford, England) · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transgenesis promises a powerful means for assessing gene function during amphibian limb regeneration. This approach is complicated, however, by the need for embryonic appendage development to proceed unimpeded despite the genetic alterations one wishes to test later in the context of regeneration. Achieving conditional gene regulation in this amphibian has not proved to be as straightforward as in many other systems. In this report we describe a unique method for obtaining temporal control over exogenous gene expression in the axolotl. Based on technology derived from the Escherichia coli Lac operon, uninduced transgenes are kept in a repressed state by the binding of constitutively expressed Lac repressor protein (LacI) to operator sequences within the expression construct. Addition of a lactose analog, IPTG, to the swimming water of the axolotl is sufficient for the sugar to be taken up by cells, where it binds the LacI protein, thereby inducing expression of the repressed gene. We use this system to demonstrate an in vivo role for thrombospondin-4 in limb regeneration. This inducible system will allow for systematic analysis of phenotypes at defined developmental or regenerative time points. The tight regulation and robustness of gene induction combined with the simplicity of this strategy will prove invaluable for studying many aspects of axolotl biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.