ArticleAntimicrobial agents and chemotherapy2012
DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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19 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- The global prevalence of fusidic acid resistance in clinical isolates of Staphylococcus aureus: a systematic review and meta-analysis.Antimicrobial resistance and infection control · 2021Pooled it
- Methicillin-resistant Staphylococcus aureus colonization: a three-year prospective study in a neonatal intensive care unit in Italy.PloS one · 2014Trial
- Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Molecular epidemiology of methicillin-resistantFrontiers in microbiology · 2025Review
- Diversity of clonal complex 22 methicillin-resistantFrontiers in microbiology · 2022Article
- Characterizing a Novel Staphylococcal Cassette ChromosomeAntimicrobial agents and chemotherapy · 2021Article
- Epidemiological typing of methicillin resistantInfection prevention in practice · 2021Article
- Updating Molecular Diagnostics for Detecting Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus Isolates in Blood Culture Bottles.Journal of clinical microbiology · 2019Article
- Livestock-Associated Methicillin-ResistantFrontiers in microbiology · 2019Review
- Emerging variants of methicillin-resistant Staphylococcus aureus genotypes in Kuwait hospitals.PloS one · 2018Article
- Distribution of the Most PrevalentFrontiers in microbiology · 2018Review
- Characterization of the virulence potential ofNew microbes and new infections · 2017Article
- Evaluation of HiaureusJournal of clinical and diagnostic research : JCDR · 2017Article
- Omics Approaches for the Study of Adaptive Immunity toProteomes · 2016Review
- Characterization of Methicillin-ResistantFrontiers in microbiology · 2016Article
- Coagulase-negative staphylococci.Clinical microbiology reviews · 2014Review
- Panton-Valentine leukocidin-positive Staphylococcus aureus in Ireland from 2002 to 2011: 21 clones, frequent importation of clones, temporal shifts of predominant methicillin-resistant S. aureus clones, and increasing multiresistance.Journal of clinical microbiology · 2014Article
- Article
- Extensive genetic diversity identified among sporadic methicillin-resistant Staphylococcus aureus isolates recovered in Irish hospitals between 2000 and 2012.Antimicrobial agents and chemotherapy · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One hundred seventy-five isolates representative of methicillin-resistant Staphylococcus aureus (MRSA) clones that predominated in Irish hospitals between 1971 and 2004 and that previously underwent multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were characterized by spa typing (175 isolates) and DNA microarray profiling (107 isolates). The isolates belonged to 26 sequence type (ST)-SCCmec types and subtypes and 35 spa types. The array assigned all isolates to the correct MLST clonal complex (CC), and 94% (100/107) were assigned an ST, with 98% (98/100) correlating with MLST. The array assigned all isolates to the correct SCCmec type, but subtyping of only some SCCmec elements was possible. Additional SCCmec/SCC genes or DNA sequence variation not detected by SCCmec typing was detected by array profiling, including the SCC-fusidic acid resistance determinant Q6GD50/fusC. Novel SCCmec/SCC composite islands (CIs) were detected among CC8 isolates and comprised SCCmec IIA-IIE, IVE, IVF, or IVg and a ccrAB4-SCC element with 99% DNA sequence identity to SCC(M1) from ST8/t024-MRSA, SCCmec VIII, and SCC-CI in Staphylococcus epidermidis. The array showed that the majority of isolates harbored one or more superantigen (94%; 100/107) and immune evasion cluster (91%; 97/107) genes. Apart from fusidic acid and trimethoprim resistance, the correlation between isolate antimicrobial resistance phenotype and the presence of specific resistance genes was ≥97%. Array profiling allowed high-throughput, accurate assignment of MRSA to CCs/STs and SCCmec types and provided further evidence of the diversity of SCCmec/SCC. In most cases, array profiling can accurately predict the resistance phenotype of an isolate.
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