ArticleAntimicrobial agents and chemotherapy2012
Antiparasitic Effect of Vitamin B12 on Trypanosoma cruzi.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Neglected tropical diseases and vitamin B12: a review of the current evidence.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2018Pooled it
- Optimization and biological validation of anBiology methods & protocols · 2021Article
- Using prodigiosin against some gram-positive and gram-negative bacteria andThe journal of venomous animals and toxins including tropical diseases · 2019Article
- Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis inFrontiers in cellular and infection microbiology · 2019Review
- Anti-parasitic effect of vitamin C alone and in combination with benznidazole against Trypanosoma cruzi.PLoS neglected tropical diseases · 2018Article
- The superfamily keeps growing: Identification in trypanosomatids of RibJ, the first riboflavin transporter family in protists.PLoS neglected tropical diseases · 2017Article
- Mode of Action of the Sesquiterpene Lactones Psilostachyin and Psilostachyin C on Trypanosoma cruzi.PloS one · 2016Article
- The Combination of Vitamin K3 and Vitamin C Has Synergic Activity against Forms of Trypanosoma cruzi through a Redox Imbalance Process.PloS one · 2015Article
- Trypanocidal activity of thioamide-substituted imidazoquinolinone: electrochemical properties and biological effects.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Natural terpenoids from Ambrosia species are active in vitro and in vivo against human pathogenic trypanosomatids.PLoS neglected tropical diseases · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nutritional characteristic of trypanosomatid protozoa is that they need a heme compound as a growth factor. Because of the cytotoxic activity of heme and its structural similarity to cobalamins, we have investigated the in vitro and in vivo effect of vitamin B(12) (or cyanocobalamin) on the different forms of Trypanosoma cruzi. Cyanocobalamin showed a marked antiparasitic activity against epimastigotes (50% inhibitory concentration [IC(50)], 2.42 μM), amastigotes (IC(50), 10.69 μM), and trypomastigotes (IC(50), 9.46 μM). Anti-epimastigote and -trypomastigote values were 1.7 to 4 times lower than those obtained with the reference drug benznidazole (Bnz). We also found that B(12) and hemin do not interact with each other in their modes of action. Our results show that B(12) increases intracellular oxidative activity and stimulates both superoxide dismutase (50%) and ascorbate peroxidase (20%) activities, while the activity of trypanothione reductase was not modified. In addition, we found that the antioxidants dithiothreitol and ascorbic acid increase the susceptibility of the parasite to the cytotoxic action of B(12). We propose that vitamin B(12) exerts its growth-inhibitory effect through the generation of reactive oxygen species. In an in vivo assay, a significant reduction in the number of circulating parasites was found in T. cruzi-infected mice treated with cyanocobalamin and ascorbic acid. The reduction of parasitemia in benznidazole-treated mice was improved by the addition of these vitamins. According to our results, a combination of B(12) and Bnz should be further investigated due to its potential as a new therapeutic modality for the treatment of Chagas' disease.
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