ArticleSoil biology & biochemistry2012
A field method to store samples from temperate mountain grassland soils for analysis of phospholipid fatty acids.
Article in Soil biology & biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Desiccation as a suitable alternative to cold-storage of phyllosphere samples for DNA-based microbial community analyses.Scientific reports · 2025Article
- Drying as an effective method to store soil samples for DNA-based microbial community analyses: a comparative study.Scientific reports · 2024Article
- Storage of soil microbiome for application in sustainable agriculture: prospects and challenges.Environmental science and pollution research international · 2022Review
- The guanidine thiocyanate-high EDTA method for total microbial RNA extraction from severely heavy metal-contaminated soils.Microbial biotechnology · 2021Article
- Keeping it cool: Soil sample cold pack storage and DNA shipment up to 1 month does not impact metabarcoding results.Ecology and evolution · 2020Article
- Rapid Transfer of Plant Photosynthates to Soil Bacteria via Ectomycorrhizal Hyphae and Its Interaction With Nitrogen Availability.Frontiers in microbiology · 2019Article
- Effects of preservation method on canine (PeerJ · 2018Article
- Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia.Soil biology & biochemistry · 2015Article
- Input of easily available organic C and N stimulates microbial decomposition of soil organic matter in arctic permafrost soil.Soil biology & biochemistry · 2014Article
- Effects of soil organic matter properties and microbial community composition on enzyme activities in cryoturbated arctic soils.PloS one · 2014Article
- Nitrogen dynamics in Turbic Cryosols from Siberia and Greenland.Soil biology & biochemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The storage of soil samples for PLFA analysis can lead to shifts in the microbial community composition. We show here that conserving samples in RNAlater, which is already widely used to store samples for DNA and RNA analysis, proved to be as sufficient as freezing at -20 °C and preferable over storage at 4 °C for temperate mountain grassland soil. The total amount of extracted PLFAs was not changed by any storage treatment. Storage at 4 °C led to an alteration of seven out of thirty individual biomarkers, while freezing and storage in RNAlater caused changes in the amount of fungal biomarkers but had no effect on any other microbial group. We therefore suggest that RNAlater could be used to preserve soil samples for PLFA analysis when immediate extraction or freezing of samples is not possible, for example during sampling campaigns in remote areas or during transport and shipping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.