ArticleJournal of experimental botany2012
Polyphenol oxidases in Physcomitrella: functional PPO1 knockout modulates cytokinin-dependent development in the moss Physcomitrella patens.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- The Biosynthesis and Functions of Flavonoids: Recent Advances From Studies Across Land Plant Diversity.Journal of the Royal Society of New Zealand · 2026Review
- Birth and death evolution of polyphenol oxidase (PPO) gene family in Oryza species.Botanical studies · 2026Article
- Secretion-based production of prolyl-hydroxylated human type III collagen in scalable Physcomitrella photobioreactors.Plant cell reports · 2026Article
- EndophyticPlants (Basel, Switzerland) · 2025Article
- Article
- Isolation and Identification of Endophytic Bacterium B5 fromJournal of fungi (Basel, Switzerland) · 2025Article
- Flagellin FLiS improves the resistance of cotton to Verticillium wilt through the signaling pathways of salicylic acid and jasmonic acid.Frontiers in plant science · 2025Article
- Biocontrol Potential of EndophyticJournal of fungi (Basel, Switzerland) · 2024Article
- Isolation, Identification, and Biocontrol Mechanisms of EndophyticMicroorganisms · 2024Article
- The PPO family in Nicotiana tabacum is an important regulator to participate in pollination.BMC plant biology · 2024Article
- Screening, identification and evaluation of an acidophilic strain ofFrontiers in plant science · 2024Article
- Isolation and Identification of Biocontrol Bacteria againstMicroorganisms · 2023Article
- The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens inFrontiers in plant science · 2022Article
- Biocontrol Potential of EndophyticFrontiers in plant science · 2022Article
- Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of Physcomitrium patens with Botrytis cinerea.Plant molecular biology · 2021Article
- Phytohormone biosynthesis and signaling pathways of mosses.Plant molecular biology · 2021Review
- Site-directed mutagenesis around the CuA site of a polyphenol oxidase from Coreopsis grandiflora (cgAUS1).FEBS letters · 2015Article
- Article
- Isopentenyltransferase-1 (IPT1) knockout in Physcomitrella together with phylogenetic analyses of IPTs provide insights into evolution of plant cytokinin biosynthesis.Journal of experimental botany · 2014Article
- Reduced polyphenol oxidase gene expression and enzymatic browning in potato (Solanum tuberosum L.) with artificial microRNAs.BMC plant biology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyphenol oxidases (PPOs) are copper-binding enzymes of the plant secondary metabolism that oxidize polyphenols to quinones. Although PPOs are nearly ubiquitous in seed plants, knowledge on their evolution and function in other plant groups is missing. This study reports on the PPO gene family in the moss Physcomitrella patens (Hedw.) B.S.G. asan example for an early divergent plant. The P. patens PPO multigene family comprises 13 paralogues. Phylogenetic analyses suggest that plant PPOs evolved with the colonization of land and that PPO duplications within the monophyletic P. patens paralogue clade occurred after the separation of the moss and seed plant lineages. PPO functionality was demonstrated for recombinant PPO6. P. patens was analysed for phenolic compounds and six substances were detected intracellularly by LC-MS analysis: 4-hydroxybenzoic acid, p-cumaric acid, protocatechuic acid, salicylic acid, caffeic acid, and an ester of caffeic acid. Targeted PPO1 knockout (d|ppo1) plants were generated and plants lacking PPO1 exhibited only ~30% of the wild-type PPO activity in the culture medium, thus suggesting extracellular localization of PPO1, which is in contrast to the mostly plastidic PPO localization in seed plants. Further, d|ppo1 lines formed significantly more gametophores with a reduced areal plant size, which could be related to an increase of endogenously produced cytokinins and indicates an impact of PPO1 on plant development. d|ppo1 plants were less tolerant towards applied 4-methylcatechol compared to the wild type, which suggests a role of extracellular PPO1 in establishing appropriate conditions by the removal of inhibitory extracellular phenolic compounds.
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