ArticleJournal of experimental botany2012
Contribution of the different omega-3 fatty acid desaturase genes to the cold response in soybean.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 123 citations in OpenAlex.
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- Transcriptome Analysis Reveals Metabolic Pathways and Key Genes Involved in Oleic Acid Formation of Sunflower (International journal of molecular sciences · 2025Article
- Genome-wide analysis of theFrontiers in plant science · 2025Article
- Exploring the role of FAT genes in Solanaceae species through genome-wide analysis and genome editing.The plant genome · 2024Article
- Identification of environment-insensitive genes for oil content by combination of transcriptome and genome-wide association analysis in rapeseed.Biotechnology for biofuels and bioproducts · 2024Article
- Molecular cloning and functional analysis of a plastidial ω3 desaturase fromFrontiers in microbiology · 2024Article
- Genome-Wide Characterization of TomatoPlants (Basel, Switzerland) · 2023Article
- Alternative Splicing Variation: Accessing and Exploiting in Crop Improvement Programs.International journal of molecular sciences · 2023Review
- Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance.BMC plant biology · 2023Article
- Comparative transcriptomic analysis of germinating rice seedlings to individual and combined anaerobic and cold stress.BMC genomics · 2023Article
- Phytohormones regulate the non-redundant response of ω-3 fatty acid desaturases to low temperatures in Chorispora bungeana.Scientific reports · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance.Molecular biology reports · 2022Review
- Genome-Wide Characterization and Expression Analysis of Fatty acid Desaturase Gene Family in Poplar.International journal of molecular sciences · 2022Article
- Increase in alpha-linolenic acid content by simultaneous expression of fatty acid metabolism genes in Sesame (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Overexpression ofGenes · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Changes in the fatty acid composition of pine needle lipids under the aluminum smelter emissions.Ecotoxicology (London, England) · 2021Article
- Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated.BMC genomics · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study analysed the contribution of each omega-3 desaturase to the cold response in soybean. Exposure to cold temperatures (5 °C) did not result in great modifications of the linolenic acid content in leaf membrane lipids. However, an increase in the GmFAD3A transcripts was observed both in plant leaves and soybean cells whereas no changes in GmFAD3B or GmFAD3C expression levels were detected. This increase was reversible and accompanied by the accumulation of an mRNA encoding a truncated form of GmFAD3A (GmFAD3A-T), which originated from alternative splicing of GmFAD3A in response to cold. When the expression of plastidial omega-3 desaturases was analysed, a transient accumulation of GmFAD7-2 mRNA was detected upon cold exposure in mature soybean trifoliate leaves while GmFAD7-1 transcripts remained unchanged. No modification of the GmFAD8-1 and GmFAD8-2 transcripts was observed. The functionality of GmFAD3A, GmFAD3B, GmFAD3C and GmFAD3A-T was examined by heterologous expression in yeast. No activity was detected with GmFAD3A-T, consistent with the absence of one of the His boxes necessary for desaturase activity. The linolenic acid content of Sacharomyces cerevisiae cells overexpressing GmFAD3A or GmFAD3B was higher when the cultures were incubated at cooler temperatures, suggesting that reticular desaturases of the GmFAD3 family, and more specifically GmFAD3A, may play a role in the cold response, even in leaves. The data point to a regulatory mechanism of omega-3 fatty acid desaturases in soybean affecting specific isoforms in both the plastid and the endoplasmic reticulum to maintain appropriate levels of linolenic acid under low temperature conditions.
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