ArticleJournal of bacteriology2012
Reduced Na+ affinity increases turnover of Salmonella enterica serovar Typhimurium MelB.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Structure and allostery in major facilitator superfamily symport mechanisms.Acta crystallographica. Section D, Structural biology · 2026Article
- Article
- Allosteric effects of the coupling cation in melibiose transporter MelB.bioRxiv : the preprint server for biology · 2025Article
- Distinct roles of the major binding residues in the cation-binding pocket of MelB.bioRxiv : the preprint server for biology · 2024Article
- Mobile barrier mechanisms for NaeLife · 2024Article
- Mobile barrier mechanisms for NabioRxiv : the preprint server for biology · 2023Article
- Substrate-binding guides individual melibiose permeases MelB to structurally soften and to destabilize cytoplasmic middle-loop C3.Structure (London, England : 1993) · 2023Article
- Molecular Basis for the Cation Selectivity of Salmonella typhimurium Melibiose Permease.Journal of molecular biology · 2022Article
- X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB.Communications biology · 2021Article
- Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.BMC biology · 2018Article
- Thermodynamic cooperativity of cosubstrate binding and cation selectivity ofThe Journal of general physiology · 2017Article
- Effect of Detergents on Galactoside Binding by Melibiose Permeases.Biochemistry · 2015Article
- A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen.Scientific reports · 2015Article
- Survival of Escherichia coli cells on solid copper surfaces is increased by glutathione.Applied and environmental microbiology · 2014Article
- Suppression of conformation-compromised mutants of Salmonella enterica serovar Typhimurium MelB.Journal of bacteriology · 2014Article
- Structure-based mechanism for Na(+)/melibiose symport by MelB.Nature communications · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The melibiose permease of Salmonella enterica serovar Typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+). Bioinformatics and mutational analyses indicate that a conserved Gly117 (helix IV) is a component of the Na(+)-binding site. In this study, Gly117 was mutated to Ser, Asn, or Cys. All three mutations increase the maximum rate (V(max)) for melibiose transport in Escherichia coli DW2 and greatly decrease Na(+) affinity, indicating that intracellular release of Na(+) is facilitated. Rapid melibiose transport, particularly by the G117N mutant, triggers osmotic lysis in the lag phase of growth. The findings support the previous conclusion that Gly117 plays an important role in cation binding and translocation. Furthermore, a spontaneous second-site mutation (P148L between loop(4-5) and helix V) in the G117C mutant prevents cell lysis. This mutation significantly decreases V(max) with little effect on cosubstrate binding in G117C, G117S, and G117N mutants. Thus, the P148L mutation specifically inhibits transport velocity and thereby blocks the lethal effect of elevated melibiose transport in the Gly117 mutants.
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