ArticleJournal of bacteriology2012
Phosphoketolase pathway for xylose catabolism in Clostridium acetobutylicum revealed by 13C metabolic flux analysis.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 73 citations in OpenAlex.
- Electrostatic Engineering of Phosphoketolase Enhances Activity on Small Nonphosphorylated Sugars and Improves Cell-Free ATP Regeneration from Inexpensive C2-Substrates.The journal of physical chemistry. B · 2026Article
- Pseudomonas aeruginosa Relies on a Phosphoketolase to Support Anaerobic Survival Under Reductive Stress.Molecular microbiology · 2026Article
- Cell-Free Reaction System for ATP Regeneration from d-Fructose.ACS synthetic biology · 2025Article
- Halosquirtibacter laminarini gen. nov., sp. nov. and Halosquirtibacter xylanolyticus sp. nov., marine anaerobic laminarin and xylan degraders in the phylum Bacteroidota.Scientific reports · 2024Article
- Alleviation of Carbon Catabolite Repression throughApplied and environmental microbiology · 2023Article
- Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering.Metabolic engineering communications · 2022Review
- A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803.Scientific reports · 2020Article
- Enhancing xylose and glucose utilization as well as solvent production using a simplified three-electrode potentiostat system during Clostridium fermentation.Journal of industrial microbiology & biotechnology · 2020Article
- Ferrous-Iron-Activated Transcriptional Factor AdhR Regulates Redox Homeostasis inApplied and environmental microbiology · 2020Article
- Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.Microbial biotechnology · 2020Review
- Control of solvent production by sigma-54 factor and the transcriptional activator AdhR in Clostridium beijerinckii.Microbial biotechnology · 2020Article
- The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase.The Journal of biological chemistry · 2020Article
- Medium-Chain Fatty Acid Synthesis by "Applied and environmental microbiology · 2020Article
- Biochemical routes for uptake and conversion of xylose by microorganisms.Biotechnology for biofuels · 2020Review
- The Butyrogenic and Lactic Bacteria of the Gut Microbiota Determine the Outcome of Allogenic Hematopoietic Cell Transplant.Frontiers in microbiology · 2020Review
- Engineering microbial pathways for production of bio-based chemicals from lignocellulosic sugars: current status and perspectives.Biotechnology for biofuels · 2020Review
- From Escherichia coli mutant 13C labeling data to a core kinetic model: A kinetic model parameterization pipeline.PLoS computational biology · 2019Article
- Genomic insights from Monoglobus pectinilyticus: a pectin-degrading specialist bacterium in the human colon.The ISME journal · 2019Article
- EMUlator: An Elementary Metabolite Unit (EMU) Based Isotope Simulator Enabled by Adjacency Matrix.Frontiers in microbiology · 2019Article
- Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis.Biotechnology for biofuels · 2019Review
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Solvent-producing clostridia are capable of utilizing pentose sugars, including xylose and arabinose; however, little is known about how pentose sugars are catabolized through the metabolic pathways in clostridia. In this study, we identified the xylose catabolic pathways and quantified their fluxes in Clostridium acetobutylicum based on [1-(13)C]xylose labeling experiments. The phosphoketolase pathway was found to be active, which contributed up to 40% of the xylose catabolic flux in C. acetobutylicum. The split ratio of the phosphoketolase pathway to the pentose phosphate pathway was markedly increased when the xylose concentration in the culture medium was increased from 10 to 20 g liter(-1). To our knowledge, this is the first time that the in vivo activity of the phosphoketolase pathway in clostridia has been revealed. A phosphoketolase from C. acetobutylicum was purified and characterized, and its activity with xylulose-5-P was verified. The phosphoketolase was overexpressed in C. acetobutylicum, which resulted in slightly increased xylose consumption rates during the exponential growth phase and a high level of acetate accumulation.
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